Susceptibility of Neisseria gonorrhoeae DNA to cleavage by restriction endonuclease KpnI.

Annales de l'Institut Pasteur. Microbiologie ·Vol. 136A ·No. 3 ·1985-00-00 ·Pages 329-38

Prère MF, Fayet O

Abstract

We compared the susceptibility of Escherichia coli and Neisseria gonorrhoeae DNA to cleavage by KpnI and found that KpnI has a lower affinity for gonococcal DNA. Site-specific methylation is suggested as the cause of this altered affinity.

MeSH Terms

DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Restriction Enzymes/metabolism DNA, Bacterial/metabolism Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics Neisseria gonorrhoeae/genetics Plasmids

Chemicals

DNA, Bacterial DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GGTACC-specific type II deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Prère M F

Fayet O

Article Info

Journal

Annales de l'Institut Pasteur. Microbiologie

Abbr.

Ann Inst Pasteur Microbiol (1985)

DOI

10.1016/s0769-2609(85)80095-8

Published

1985-00-00

Pages

329-38

Language

English

Region

Netherlands

NLM ID

8503044

Subset

External Links

PubMed source DOI: 10.1016/s0769-2609(85)80095-8

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Susceptibility of Neisseria gonorrhoeae DNA to cleavage by restriction endonuclease KpnI.

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