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PMID: 2994058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 17 ·1985-09-00 ·Pages 6001-5

Dascal N, Lotan I, Gillo B, Lester HA, Lass Y

Abstract

In Xenopus laevis oocytes, adenosine and other purinergic agonists induce a K+-conductance increase that is fully mimicked by intracellular application of cAMP. Acetylcholine suppresses the K+-conductance increase caused by adenosine, by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, or by intracellular injection of cAMP. This effect of acetylcholine is not mimicked by intracellular injection of Ca2+ or of the Ca-mobilizing agent inositol 1,4,5-trisphosphate. However, adenosine and cAMP responses are inhibited by 4 beta-phorbol 12,13-dibutyrate and 4 beta-phorbol 12-myristate 13-acetate. These results suggest that, in Xenopus oocytes, the muscarinic inhibition of purinergic and cAMP responses is mediated through the activation of the phospholipid-dependent, Ca-activated protein kinase (protein kinase C).

MeSH Terms
Acetylcholine/pharmacology Adenosine/antagonists & inhibitors Animals Cyclic AMP/antagonists & inhibitors Electric Conductivity Female Oocytes/drug effects,physiology Phorbol Esters/pharmacology Phorbols/pharmacology Potassium/physiology Protein Kinase C Protein Kinases/physiology Receptors, Cell Surface/drug effects Receptors, Purinergic Xenopus laevis
Chemicals
Phorbol Esters Phorbols Receptors, Cell Surface Receptors, Purinergic Cyclic AMP Protein Kinases Protein Kinase C Adenosine Acetylcholine Potassium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dascal N
Lotan I
Gillo B
Lester H A
Lass Y
References (29)
29 references, click to expand
  1. Acetylcholine receptors in the oocyte membrane.
    Nature. 1977 Dec 22-29;270(5639):739-41 PMID: 22819
  2. Possible role for cyclic nucleotides and phosphorylated membrane proteins in postsynaptic actions of neurotransmitters.
    Nature. 1976 Mar 11;260(5547):101-8 PMID: 176592
  3. Role of cyclic nucleotides in excitable cells.
    Annu Rev Physiol. 1980;42:629-41 PMID: 6996602
  4. Types of muscarinic response in Xenopus oocytes.
    Life Sci. 1980 Oct 13;27(15):1423-8 PMID: 7442451
  5. Mechanisms of slow postsynaptic potentials.
    Nature. 1981 Jun 18;291(5816):539-44 PMID: 6113545
  6. The molecular basis of neurotransmission at the muscarinic receptor.
    Biochem Pharmacol. 1981 Nov 1;30(21):2887-91 PMID: 6274353
  7. Bimodal regulation of adenylate cyclase.
    FEBS Lett. 1982 Feb 22;138(2):157-63 PMID: 6121719
  8. Characterization of the soluble cyclic nucleotide phosphodiesterases in Xenopus laevis oocytes. Evidence for a calmodulin-dependent enzyme.
    Biochim Biophys Acta. 1982 Feb 18;701(2):253-9 PMID: 6280770
  9. Adenosine-induced slow ionic currents in the Xenopus oocyte.
    Nature. 1982 Aug 5;298(5874):572-4 PMID: 6285198
  10. Molecular biology of learning: modulation of transmitter release.
    Science. 1982 Oct 29;218(4571):433-43 PMID: 6289442
  11. Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
    J Physiol. 1982 Jul;328:143-70 PMID: 7131311
  12. Muscarinic cholinergic receptor-mediated activation of phosphodiesterase.
    Mol Pharmacol. 1982 Sep;22(2):310-9 PMID: 6183568
  13. Identification and properties of cyclic nucleotide phosphodiesterases.
    Mol Cell Endocrinol. 1982 Nov-Dec;28(3):387-410 PMID: 6185373
  14. Calcium channel modulation by neurotransmitters, enzymes and drugs.
    Nature. 1983 Feb 17-23;301(5901):569-74 PMID: 6131381
  15. The involvement of a calmodulin-dependent phosphodiesterase in the negative control of carbamylcholine on cyclic AMP levels in dog thyroid slices.
    FEBS Lett. 1983 Jan 24;151(2):273-6 PMID: 6187601
  16. Time course of the increase in the myocardial slow inward current after a photochemically generated concentration jump of intracellular cAMP.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2395-9 PMID: 6300914
  17. The role of cyclic nucleotide phosphodiesterase in the inhibition of cyclic AMP accumulation by carbachol and phosphatidate.
    J Cyclic Nucleotide Res. 1982;8(6):395-408 PMID: 6193153
  18. The role of protein kinase C in cell surface signal transduction and tumour promotion.
    Nature. 1984 Apr 19-25;308(5961):693-8 PMID: 6232463
  19. Phospholipid-sensitive Ca2+-dependent protein kinase: a major protein phosphorylation system.
    Mol Cell Endocrinol. 1984 May;35(2-3):65-73 PMID: 6203790
  20. Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'-cyclic monophosphate.
    J Physiol. 1984 Jul;352:551-74 PMID: 6086916
  21. Protein kinase C activation of physiological processes in human neutrophils at vanishingly small cytosolic Ca2+ levels.
    Nature. 1984 Aug 23-29;310(5979):691-3 PMID: 6236373
  22. Influence of a tumor-promoting phorbol ester on the electrical response of B-cells to glucose and glyburide.
    Mol Pharmacol. 1984 Sep;26(2):267-71 PMID: 6434939
  23. Progesterone inhibition of Xenopus oocyte adenylate cyclase is not mediated via the Bordetella pertussis toxin substrate.
    Mol Pharmacol. 1984 Nov;26(3):526-31 PMID: 6541752
  24. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  25. Phorbol ester and diacylglycerol mimic growth factors in raising cytoplasmic pH.
    Nature. 1984 Nov 22-28;312(5992):371-4 PMID: 6095096
  26. Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
    Nature. 1985 Jan 10-18;313(5998):141-3 PMID: 2578219
  27. Enhancement of calcium current in Aplysia neurones by phorbol ester and protein kinase C.
    Nature. 1985 Jan 24-30;313(6000):313-6 PMID: 2578617
  28. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
    J Morphol. 1972 Feb;136(2):153-79 PMID: 4109871
  29. The role of hormone receptors and GTP-regulatory proteins in membrane transduction.
    Nature. 1980 Mar 6;284(5751):17-22 PMID: 6101906
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.17.6001
Published
1985-09-00
Pages
6001-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390682
Subset
IM
Grants
NIGMS NIH HHS · GM29836 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.17.6001 PMC Full Text View journal details
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