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PMID: 2994040 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

[4Fe-4S]-cluster-depleted Azotobacter vinelandii ferredoxin I: a new 3Fe iron-sulfur protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 17 ·1985-09-00 ·Pages 5661-5

Stephens PJ, Morgan TV, Devlin F, Penner-Hahn JE, Hodgson KO, Scott RA, Stout CD, Burgess BK

Abstract

Fe(CN)6(-3) oxidation of the aerobically isolated 7Fe Azotobacter vinelandii ferredoxin I, (7Fe)FdI, is a degradative reaction. Destruction of the [4Fe-4S] cluster occurs first, followed by destruction of the [3Fe-3S] cluster. At a Fe(CN)6(-3)/(7Fe)FdI concentration ratio of 20, the product is a mixture of apoprotein and protein containing only a [3Fe-3S] cluster, (3Fe)FdI. This protein mixture, after partial purification, has been characterized by absorption, CD, magnetic CD, and EPR and Fe x-ray absorption spectroscopies. EPR and magnetic CD spectra provide strong evidence that the [3Fe-3S] cluster in (3Fe)FdI is essentially identical in structure to that in (7Fe)FdI. Analysis of the extended x-ray absorption fine structure (EXAFS) of (3Fe)FdI finds Fe scattering at an average Fe...Fe distance of approximately equal to 2.7 A. The structure of the oxidized [3Fe-3S] cluster in solutions of oxidized (3Fe)FdI, and, by extension, of oxidized (7Fe)FdI, is thus different from that obtained by x-ray crystallography on oxidized (7Fe)FdI. Possible interpretations of this result are discussed.

MeSH Terms
Azotobacter/analysis Circular Dichroism Electron Spin Resonance Spectroscopy Ferredoxins/analysis Ferricyanides Iron-Sulfur Proteins/analysis Metalloproteins/analysis Oxidation-Reduction Spectrophotometry Spectrophotometry, Atomic
Chemicals
Ferredoxins Ferricyanides Iron-Sulfur Proteins Metalloproteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Stephens P J
Morgan T V
Devlin F
Penner-Hahn J E
Hodgson K O
Scott R A
Stout C D
Burgess B K
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.17.5661
Published
1985-09-00
Pages
5661-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390611
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.17.5661 PMC Full Text View journal details
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