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PMID: 2993338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus.

Journal of virological methods ·Vol. 11 ·No. 3 ·1985-07-00 ·Pages 265-9

Binns MM, Boursnell ME, Foulds IJ, Brown TD

Abstract

An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.

MeSH Terms

Cloning, Molecular Coronaviridae/genetics DNA/biosynthesis Genes, Viral Infectious bronchitis virus/genetics Nucleic Acid Hybridization Oligodeoxyribonucleotides/biosynthesis Plasmids

Chemicals

Oligodeoxyribonucleotides DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Binns M M

Boursnell M E

Foulds I J

Brown T D

References (9)

9 references, click to expand

Molecular cloning of the 3'-proximal third of Sendai virus genome.
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5213-6 PMID: 6310565
A simple and very efficient method for generating cDNA libraries.
Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
A rapid boiling method for the preparation of bacterial plasmids.
Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV.
J Gen Virol. 1985 Apr;66 ( Pt 4):719-26 PMID: 2984314
A leader sequence is present on mRNA A of avian infectious bronchitis virus.
J Gen Virol. 1984 Aug;65 ( Pt 8):1437-42 PMID: 6086827
Studies on transformation of Escherichia coli with plasmids.
J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
Sequence of the membrane protein gene from avian coronavirus IBV.
Virus Res. 1984;1(4):303-13 PMID: 6099661
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397

Article Info

Journal

Journal of virological methods

Abbr.

J Virol Methods

ISSN

0166-0934

DOI

10.1016/0166-0934(85)90116-8

Published

1985-07-00

Pages

265-9

Language

English

Region

Netherlands

NLM ID

8005839

PMCID

PMC7119584

Subset

External Links

PubMed source DOI: 10.1016/0166-0934(85)90116-8 PMC Full Text View journal details

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The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus.

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