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PMID: 29930094 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates.

Science (New York, N.Y.) ·Vol. 361 ·No. 6400 ·2018-00-27 ·Pages 412-415

Cho WK, Spille JH, Hecht M, Lee C, Li C, Grube V, Cisse II

Abstract

Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.

MeSH Terms
Animals Cells, Cultured Chromatin/metabolism Embryonic Stem Cells/metabolism Enhancer Elements, Genetic Gene Expression Regulation Luminescent Proteins/analysis,chemistry Mediator Complex/analysis,chemistry,metabolism Mice Molecular Imaging/methods RNA Polymerase II/analysis,chemistry,metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Chromatin Dendra2 protein, Dendronephthya Luminescent Proteins Mediator Complex Transcription Factors RNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cho Won-Ki ORCID
Department of Physics, MIT, Cambridge, MA 02139, USA.
Spille Jan-Hendrik ORCID
Department of Physics, MIT, Cambridge, MA 02139, USA.
Hecht Micca ORCID
Department of Physics, MIT, Cambridge, MA 02139, USA.
Lee Choongman
Department of Physics, MIT, Cambridge, MA 02139, USA.
Li Charles ORCID
Department of Biology, MIT, Cambridge, MA 02139, USA. | Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA.
Grube Valentin
Department of Physics, MIT, Cambridge, MA 02139, USA. | Department of Physics, LMU Munich, Geschwister Scholl Platz 1, 80539 Munich, Germany.
Cisse Ibrahim I ORCID
Department of Physics, MIT, Cambridge, MA 02139, USA.
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.aar4199
Published
2018-00-27
Epub
2018-00-21
Pages
412-415
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC6543815
Subset
IM
Grants
NCI NIH HHS · DP2 CA195769 · United States
NIGMS NIH HHS · T32 GM087237 · United States
Corrections
CommentIn
PMID: 29993031
CommentIn
PMID: 30013225
CommentIn
PMID: 30049863
External Links
PubMed source DOI: 10.1126/science.aar4199 PMC Full Text View journal details
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