Digitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes.

The Biochemical journal ·Vol. 228 ·No. 3 ·1985-06-15 ·Pages 757-60

Lindros KO, Penttilä KE

Abstract

Intact rat liver cells from the perivenous region were isolated by collagenase perfusion after first destroying the periportal region by a brief portal infusion of digitonin. Periportal cells were isolated after retrograde digitonin infusion. Significantly higher alanine aminotransferase, gamma-glutamyltransferase and lactate dehydrogenase activities and lower glutamate dehydrogenase and pyruvate kinase activities in periportal than in perivenous cells demonstrate marked separation. The high yield allows further characterization in vitro of the cell populations.

MeSH Terms

Animals Cell Separation/methods Digitonin Liver/cytology,enzymology Male Microbial Collagenase Perfusion Rats

Chemicals

Microbial Collagenase Digitonin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lindros K O

Penttilä K E

References (16)

16 references, click to expand

High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.
J Cell Biol. 1969 Dec;43(3):506-20 PMID: 4900611
Subdivision of hexagonal liver lobules into a structural and functional unit; role in hepatic physiology and pathology.
Anat Rec. 1954 May;119(1):11-33 PMID: 13180999
Fluorometric assay of proteins in the nanogram range.
Arch Biochem Biophys. 1973 Mar;155(1):213-20 PMID: 4736505
A simple and inexpensive membrane "lung" for small organ perfusion.
J Lipid Res. 1974 Mar;15(2):182-6 PMID: 4832759
Ficoll density separation of enzymatically isolated rat liver cells.
Acta Hepatogastroenterol (Stuttg). 1974 Aug;21(4):245-53 PMID: 4375389
Isolation of centrolobular and perilobular hepatocytes after phenobarbital treatment.
J Cell Biol. 1975 Jul;66(1):23-41 PMID: 167029
Preparation of isolated rat liver cells.
Methods Cell Biol. 1976;13:29-83 PMID: 177845
Subcellular distribution of enzymes determined by rapid digitonin fractionation of isolated hepatocytes.
Biochem J. 1980 Feb 15;186(2):423-9 PMID: 7378059
Functional implications of liver cell heterogeneity.
Gastroenterology. 1981 Feb;80(2):393-403 PMID: 7004997
Partial separation and biochemical characteristics of periportal and perivenous hepatocytes from rat liver.
Eur J Biochem. 1981 Sep 1;118(3):591-7 PMID: 7028482
Functional hepatocellular heterogeneity.
Hepatology. 1982 May-Jun;2(3):385-95 PMID: 7042508
Characterisation of hepatocyte sub-populations generated by centrifugal elutriation.
Eur J Biochem. 1983 Aug 15;134(3):539-45 PMID: 6411467
Selective isolation of intact periportal or perivenous hepatocytes by antero- or retrograde collagenase gradient perfusion.
Liver. 1983 Jun;3(3):131-9 PMID: 6312242
The effect of chronic ethanol ingestion on ethanol metabolizing enzymes in isolated periportal and perivenous rat hepatocytes.
Hepatology. 1984 Sep-Oct;4(5):862-6 PMID: 6383997
Digitonin perfusion of rat liver. A new approach in the study of intra-acinar and intracellular compartmentation in the liver.
Biochem J. 1985 Feb 15;226(1):289-97 PMID: 3977871
Regulation of liver pyruvate kinase and the phosphoenolpyruvate crossroads.
Eur J Biochem. 1970 Mar 1;13(1):45-54 PMID: 4314710

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2280757

Published

1985-06-15

Pages

757-60

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1145048

Subset

External Links

PubMed source DOI: 10.1042/bj2280757 PMC Full Text View journal details

Analysis Services

Digitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes.

Contact

WeChat Official Account