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PMID: 2987961 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that ribosomal protein S10 itself is a cellular component necessary for transcription antitermination by phage lambda N protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 12 ·1985-06-00 ·Pages 4070-4

Das A, Ghosh B, Barik S, Wolska K

Abstract

Bacteriophage lambda N gene product acts to modify host RNA polymerase allowing the formation of a termination-resistant transcription apparatus. Previous studies have demonstrated that the nusE71 mutation that has altered the ribosomal protein S10 prevents N action in vivo. Using a coupled transcription-translation system, we demonstrate here that purified S10 protein as well as the 30S ribosomal subunit is sufficient to restore N activity in the nusE mutant extract, allowing antitermination of Rho-dependent and Rho-independent terminators. This provides direct biochemical evidence that the S10 protein itself is one of the cellular components necessary for the formation of an antitermination apparatus.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda/genetics Cytoplasm/physiology Escherichia coli/genetics Mutation Plasmids Ribosomal Proteins/physiology Transcription Factors/physiology Transcription, Genetic Viral Proteins/physiology
Chemicals
Bacterial Proteins Ribosomal Proteins Transcription Factors Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Das A
Ghosh B
Barik S
Wolska K
References (32)
32 references, click to expand
  1. Physical and functional heterogeneity of E. coli ribosomes.
    Cold Spring Harb Symp Quant Biol. 1969;34:17-24 PMID: 4909497
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components.
    J Biol Chem. 1973 Aug 25;248(16):5720-30 PMID: 4579428
  4. Gene N regulator function of phage lambda immun21: evidence that a site of N action differs from a site of N recognition.
    J Mol Biol. 1973 Dec 25;81(4):505-16 PMID: 4778807
  5. Subunit I of G beta replicase and 30 S ribosomal protein S1 of Escherichia coli. Evidence for the identity of the two proteins.
    J Biol Chem. 1974 May 25;249(10):3314-6 PMID: 4208476
  6. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534-8 PMID: 4601822
  7. Altered reading of genetic signals fused to the N operon of bacteriophage lambda: genetic evidence for modification of polymerase by the protein product of the N gene.
    J Mol Biol. 1974 Oct 15;89(1):33-48 PMID: 4613856
  8. Evidence of read-through at the termination signal for transcription of the trp operon.
    Virology. 1976 Mar;70(1):181-4 PMID: 1258377
  9. Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant.
    Virology. 1976 Aug;73(1):119-27 PMID: 785802
  10. Transcription termination sites in the major leftward operon of coliphage lambda.
    Virology. 1978 Jul 15;88(2):252-62 PMID: 695328
  11. Coliphage lambdanutL-: a unique class of mutants defective in the site of gene N product utilization for antitermination of leftward transcription.
    J Mol Biol. 1978 Sep 5;124(1):195-221 PMID: 361968
  12. Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome.
    J Bacteriol. 1979 Jan;137(1):584-94 PMID: 368032
  13. Specificity of the bacteriophage lambda N gene product (pN): nut sequences are necessary and sufficient for antitermination by pN.
    Cell. 1979 Dec;18(4):1145-51 PMID: 160286
  14. A biochemical assay for the transcription-antitermination function of the coliphage lambda N gene product.
    Gene. 1980 Jun;10(1):17-25 PMID: 6157602
  15. Evidence that ribosomal protein S10 participates in control of transcription termination.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1115-8 PMID: 6453343
  16. Ribosomal proteins S3, S6, S8 and S10 of Escherichia coli localized on the external surface of the small subunit by immune electron microscopy.
    J Mol Biol. 1981 Jan 5;145(1):193-214 PMID: 6167721
  17. The nusA gene protein of Escherichia coli. Its identification and a demonstration that it interacts with the gene N transcription anti-termination protein of bacteriophage lambda.
    J Mol Biol. 1981 Mar 25;147(1):11-23 PMID: 6455533
  18. Regulation of the S10 ribosomal protein operon in E. coli: nucleotide sequence at the start of the operon.
    Cell. 1981 Oct;26(2 Pt 2):205-11 PMID: 7037196
  19. Suppression of transcription termination by phage lambda.
    Science. 1982 May 28;216(4549):946-51 PMID: 6281888
  20. Translational coupling at an intercistronic boundary of the Escherichia coli galactose operon.
    Cell. 1982 Oct;30(3):865-71 PMID: 6754091
  21. Escherichia coli nusB mutations that suppress nusA1 exhibit lambda N specificity.
    J Mol Biol. 1983 Jul 25;168(1):73-85 PMID: 6224023
  22. lambda mutation in the Escherichia coli rho gene that inhibits the N protein activity of phage lambda.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5530-4 PMID: 6225121
  23. Architecture of prokaryotic ribosomes.
    Annu Rev Biochem. 1983;52:35-65 PMID: 6351726
  24. Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coli.
    Cell. 1983 Oct;34(3):979-88 PMID: 6354472
  25. The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli.
    Nucleic Acids Res. 1984 Apr 11;12(7):3333-42 PMID: 6326058
  26. Formation of termination-resistant transcription complex at phage lambda nut locus: effects of altered translation and a ribosomal mutation.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3612-6 PMID: 6233610
  27. The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.
    Nucleic Acids Res. 1984 Jun 25;12(12):4977-85 PMID: 6330693
  28. Molecular cloning and nucleotide sequencing of the nusB gene of E. coli.
    Nucleic Acids Res. 1984 Jun 25;12(12):4987-95 PMID: 6330694
  29. Transcription of the S10 ribosomal protein operon is regulated by an attenuator in the leader.
    Cell. 1983 May;33(1):241-8 PMID: 6380754
  30. Transcription antitermination in vitro by lambda N gene product: requirement for a phage nut site and the products of host nusA, nusB, and nusE genes.
    Cell. 1984 Aug;38(1):165-73 PMID: 6088061
  31. nusB: a protein factor necessary for transcription antitermination in vitro by phage lambda N gene product.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6305-9 PMID: 6093096
  32. Structure and function of the bacterial ribosome.
    Annu Rev Biochem. 1972;41(10):377-408 PMID: 4570961
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.12.4070
Published
1985-06-00
Pages
4070-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397936
Subset
IM
Grants
NIGMS NIH HHS · GM28946 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.12.4070 PMC Full Text View journal details
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