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PMID: 2987791 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA polymerase alpha and models for proofreading.

Nucleic acids research ·Vol. 13 ·No. 1 ·1985-01-11 ·Pages 261-74

Abbotts J, Loeb LA

Abstract

Using a modified system to measure fidelity at an amber site in phi X174, we have employed DNA polymerase alpha to test different mechanisms for proofreading. DNA polymerase alpha does not exhibit the characteristics of "kinetic proofreading" seen with procaryotic polymerases. Polymerase alpha shows no evidence for a "next nucleotide" effect, and added deoxynucleoside monophosphates do not alter fidelity. Pyrophosphate, which increases error rates with a procaryotic polymerase, appears to weakly improve polymerase alpha fidelity. DNA polymerase alpha does exhibit a dramatic increase in error rate in the presence of a deoxycytidine thiotriphosphate (dCTP alpha S), but this enhanced mutagenesis also occurs under conditions where kinetic proofreading should be otherwise defeated. This particular effect with dCTP alpha S appears specific for DNA polymerase alpha and is not seen with the other polymerases tested.

MeSH Terms
Animals Cattle DNA/genetics DNA Polymerase I/metabolism DNA Polymerase II/metabolism Diphosphates/pharmacology Escherichia coli/enzymology,genetics Models, Chemical Nucleic Acid Hybridization Nucleotides/pharmacology
Chemicals
Diphosphates Nucleotides DNA DNA Polymerase I DNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Abbotts J
Loeb L A
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.1.261
Published
1985-01-11
Pages
261-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340989
Subset
IM
Grants
NIA NIH HHS · AG 00057 · United States
NCI NIH HHS · CA 24845 · United States
External Links
PubMed source DOI: 10.1093/nar/13.1.261 PMC Full Text View journal details
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