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PMID: 2984244 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Detection of Epstein-Barr virus DNA in mouthwashes by hybridization.

Journal of clinical microbiology ·Vol. 21 ·No. 3 ·1985-03-00 ·Pages 353-6

Ambinder RF, Wingard JR, Burns WH, Hayward SD, Saral R, Perry HR, Santos GW, Hayward GS

Abstract

An assay for the presence of Epstein-Barr virus (EBV) DNA was developed by using a cloned EBV DNA probe. After preliminary testing showed the assay to be sensitive and specific, it was applied to 135 mouthwashes from bone marrow transplant recipients, and 21 of these tests were positive. The concentration of EBV DNA in mouthwashes in some cases was as high as 10(8) genome equivalents per ml. When compared with the lymphocyte transformation assay on the same samples, the sensitivity was 75% and the specificity was 97%. In contrast to the lymphocyte transformation assay, the hybridization was semiquantitative and yielded results in 72 h. Potential applications include monitoring the effects of various interventions, such as immunosuppressive and antiviral chemotherapy, on EBV shedding.

MeSH Terms
Bone Marrow Transplantation DNA, Viral/analysis Herpesvirus 4, Human/genetics Humans Lymphocyte Activation Mouth/microbiology Nucleic Acid Hybridization
Chemicals
DNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ambinder R F
Wingard J R
Burns W H
Hayward S D
Saral R
Perry H R
Santos G W
Hayward G S
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.21.3.353-356.1985
Published
1985-03-00
Pages
353-6
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC271662
Subset
IM
Grants
NCI NIH HHS · CA06973 · United States
NCI NIH HHS · CA15396 · United States
NCI NIH HHS · CA37314 · United States
External Links
PubMed source DOI: 10.1128/jcm.21.3.353-356.1985 PMC Full Text View journal details
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