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PMID: 2974924 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 3303-10

Parsons RL, Prasad PV, Harshey RM, Jayaram M

Abstract

The site-specific recombinase (FLP) encoded by the yeast plasmid 2 micron circle belongs to the integrase (of phage lambda) family of recombinases. The sparse homology within the members of this family contrasts with the invariance of three residues, His-396, Arg-399, and Tyr-433 (the numbers correspond to the family alignment positions), among them. We report here results on substrate recognition and catalysis by FLP proteins altered at these residues. Mutations of the conserved His and Tyr that aborted the reaction at specific steps of catalysis permitted genetic dissection of the possible biochemical steps of recombination. We provide indirect evidence that recombination by FLP proceeds through a Holliday junction intermediate.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,metabolism Bacteriophage lambda/genetics Base Sequence DNA Nucleotidyltransferases/genetics,metabolism Escherichia coli/genetics Molecular Sequence Data Mutation Plasmids Promoter Regions, Genetic Recombination, Genetic Substrate Specificity
Chemicals
Bacterial Outer Membrane Proteins DNA Nucleotidyltransferases FLP recombinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parsons R L
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Prasad P V
Harshey R M
Jayaram M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.3303-3310.1988
Published
1988-08-00
Pages
3303-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363564
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.8.3303-3310.1988 PMC Full Text View journal details
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