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PMID: 2974536 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The complete primary structure of the human snRNP E protein.

Nucleic acids research ·Vol. 16 ·No. 22 ·1988-11-25 ·Pages 10593-605

Stanford DR, Kehl M, Perry CA, Holicky EL, Harvey SE, Rohleder AM, Rehder K, Luhrmann R, Wieben ED

Abstract

The snRNP E protein is one of four "core" proteins associated with the snRNAs of the U family (U1,U2,U4,U5, and U6). Screening of a human teratoma cDNA library with a partial cDNA for a human autoimmune antigen resulted in the isolation of a cDNA clone containing the entire coding region of this snRNP core protein. Comparison of the 5' end of this cDNA with the sequences of two processed pseudogenes and primer extension data suggest that the cDNA is nearly full length. The longest open reading frame in this clone codes for a basic 92 amino acid protein which is in perfect agreement with amino acid sequence data obtained from purified E protein. The predicted sequence of this protein reveals no extensive similarity to other snRNP proteins, but contains regions of similarity to a eukaryotic ribosomal protein.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Neoplasm/genetics Genes Humans Molecular Sequence Data Protein Conformation Restriction Mapping Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Teratoma Tumor Cells, Cultured/metabolism
Chemicals
DNA, Neoplasm Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Stanford D R
Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.
Kehl M
Perry C A
Holicky E L
Harvey S E
Rohleder A M
Rehder K
Luhrmann R
Wieben E D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.22.10593
Published
1988-11-25
Pages
10593-605
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338926
Subset
IM
Grants
NIAMS NIH HHS · AR38554 · United States
NCI NIH HHS · CA09441 · United States
External Links
PubMed source DOI: 10.1093/nar/16.22.10593 PMC Full Text View journal details
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