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PMID: 2974240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The presence of heparan sulfate proteoglycans in the neuritic plaques and congophilic angiopathy in Alzheimer's disease.

The American journal of pathology ·Vol. 133 ·No. 3 ·1988-12-00 ·Pages 456-63

Snow AD, Mar H, Nochlin D, Kimata K, Kato M, Suzuki S, Hassell J, Wight TN

Abstract

Two immunocytochemical probes were used to specifically identify and localize heparan sulphate proteoglycans (HSPGs) in 17 cases of Alzheimer's disease (AD). A monoclonal (HK-102) and an affinity-purified polyclonal antibody, each recognizing specific domains on the protein core of a basement membrane-derived HSPG, localized HSPGs to the amyloid fibrils present in neuritic plaques (NPs) and congophilic angiopathy (CA) in the brains of Alzheimer's patients, with weak to no immunostaining in neurofibrillary tangles from the same tissues. HSPGs were also demonstrated in "primitive plaques," suggesting that their accumulation takes place during early stages of plaque development. Immunolocalization of HSPGs to subsets of astrocytes and neuronal cells, particularly those in close proximity to NPs and CA, suggested possible involvement of these two cell types in deposition of HS-PGs into the amyloidotic lesions. The current study not only identifies a new component (HSPGs) present in the amyloid deposits of NPs and CA but also suggests that astrocytes, neurons, or both may be involved in its deposition at these sites.

MeSH Terms
Alzheimer Disease/metabolism,pathology Amyloid/analysis Antibodies, Monoclonal Astrocytes/analysis,ultrastructure Brain/pathology,ultrastructure Brain Chemistry Chondroitin Sulfate Proteoglycans/analysis Congo Red Glycosaminoglycans/analysis Heparan Sulfate Proteoglycans Heparitin Sulfate/analysis Humans Immunoenzyme Techniques Immunohistochemistry Microscopy, Electron Neurofibrils/analysis,ultrastructure Neurons/analysis,ultrastructure Proteoglycans/analysis
Chemicals
Amyloid Antibodies, Monoclonal Chondroitin Sulfate Proteoglycans Glycosaminoglycans Heparan Sulfate Proteoglycans Proteoglycans Congo Red Heparitin Sulfate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Snow A D
Department of Pathology, University of Washington, Seattle 98195.
Mar H
Nochlin D
Kimata K
Kato M
Suzuki S
Hassell J
Wight T N
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1988-12-00
Pages
456-63
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1880818
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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