Home LiteratureArticle Details
PMID: 2971536 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation of a human gene with protein sequence similarity to human and murine int-1 and the Drosophila segment polarity mutant wingless.

The EMBO journal ·Vol. 7 ·No. 6 ·1988-06-00 ·Pages 1743-8

Wainwright BJ, Scambler PJ, Stanier P, Watson EK, Bell G, Wicking C, Estivill X, Courtney M, Boue A, Pedersen PS

Abstract

An expressed gene sequence which was identified by the isolation of a methylation free CpG island from human chromosome 7 has been cloned from a human lung cDNA library. The deduced protein sequence contains 360 amino acids and has several features of a secreted protein; it is cysteine rich with a signal peptide sequence and two potential asn-linked glycosylation sites. The protein sequence shows marked similarity with human and murine int-1 and their Drosophila homolog wingless (Dint-1). This human int-1 related protein, int-1 and Dint-1 have diverse patterns of expression, but the inferred structural similarities suggest that some of the functional characteristics of these proteins may be shared.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chromosomes, Human, Pair 7 DNA/genetics Drosophila melanogaster/genetics Genes Growth Substances/genetics Humans Lung/analysis Mice/genetics Molecular Sequence Data Protein Processing, Post-Translational Proto-Oncogene Proteins/genetics Wnt Proteins Wnt1 Protein Wnt2 Protein Zebrafish Proteins
Chemicals
Growth Substances Proto-Oncogene Proteins Wnt Proteins Wnt1 Protein Wnt2 Protein Zebrafish Proteins DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wainwright B J
Department of Biochemistry, St Mary's Hospital Medical School, London, UK.
Scambler P J
Stanier P
Watson E K
Bell G
Wicking C
Estivill X
Courtney M
Boue A
Pedersen P S
References (33)
33 references, click to expand
  1. The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless.
    Cell. 1987 Aug 14;50(4):649-57 PMID: 3111720
  2. Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome.
    Cell. 1982 Nov;31(1):99-109 PMID: 6297757
  3. Chromosome mediated gene transfer of six DNA markers linked to the cystic fibrosis locus on human chromosome seven.
    Nucleic Acids Res. 1986 Sep 25;14(18):7159-74 PMID: 3763403
  4. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  5. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  6. Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo.
    Cell. 1987 Jul 3;50(1):79-88 PMID: 3594565
  7. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  8. Prenatal diagnosis in 200 pregnancies with a 1-in-4 risk of cystic fibrosis.
    Hum Genet. 1986 Nov;74(3):288-97 PMID: 3536726
  9. A candidate for the cystic fibrosis locus isolated by selection for methylation-free islands.
    Nature. 1987 Apr 30-May 6;326(6116):840-5 PMID: 2883581
  10. Molecular cloning of sequences from wingless, a segment polarity gene in Drosophila: the spatial distribution of a transcript in embryos.
    EMBO J. 1987 Jun;6(6):1765-73 PMID: 16453776
  11. Phosphorylation fails to activate chloride channels from cystic fibrosis airway cells.
    Nature. 1987 Dec 24-31;330(6150):752-4 PMID: 2447502
  12. Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos.
    Cell. 1987 Jul 3;50(1):89-95 PMID: 3594566
  13. The int-1 proto-oncogene products are glycoproteins that appear to enter the secretory pathway.
    Mol Cell Biol. 1987 Nov;7(11):3978-84 PMID: 3323883
  14. The interaction of calmodulin with amphiphilic peptides.
    J Biol Chem. 1985 Feb 25;260(4):2527-34 PMID: 2982823
  15. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  16. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  17. Microvillar peptidase activity in amniotic fluid: possible use in the prenatal diagnosis of cystic fibrosis.
    Lancet. 1983 Feb 12;1(8320):329-31 PMID: 6130334
  18. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  19. The development of wingless, a homeotic mutation of Drosophila.
    Dev Biol. 1977 APR;56(2):227-40 PMID: 849798
  20. Linkage of cystic fibrosis to two tightly linked DNA markers: joint report from a collaborative study.
    Am J Hum Genet. 1986 Dec;39(6):681-93 PMID: 3026171
  21. Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.
    FEBS Lett. 1986 Feb 3;196(1):108-12 PMID: 2935423
  22. The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.
    EMBO J. 1985 Nov;4(11):2905-9 PMID: 2998762
  23. A retrovirus vector expressing the putative mammary oncogene int-1 causes partial transformation of a mammary epithelial cell line.
    Cell. 1986 Sep 26;46(7):1001-9 PMID: 3019559
  24. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  25. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  26. Physical and genetic analysis of cosmids from the vicinity of the cystic fibrosis locus.
    Nucleic Acids Res. 1987 May 11;15(9):3639-52 PMID: 3473444
  27. Mode of proviral activation of a putative mammary oncogene (int-1) on mouse chromosome 15.
    Nature. 1984 Jan 12-18;307(5947):131-6 PMID: 6318122
  28. Fast computer search for similar DNA sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5471-4 PMID: 6462911
  29. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
    Cell. 1987 Nov 6;51(3):503-12 PMID: 2822260
  30. A closely linked genetic marker for cystic fibrosis.
    Nature. 1985 Nov 28-Dec 4;318(6044):382-4 PMID: 3906407
  31. A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNA.
    Cell. 1985 Jan;40(1):91-9 PMID: 2981636
  32. Phenocopies induced with antisense RNA identify the wingless gene.
    Cell. 1987 Aug 14;50(4):659-63 PMID: 2440586
  33. Two proto-oncogenes implicated in mammary carcinogenesis, int-1 and int-2, are independently regulated during mouse development.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7806-10 PMID: 2429320
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-06-00
Pages
1743-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457162
Subset
IM
Databases
GENBANK
X07876
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2