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PMID: 2968347 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Preferential expression of fibronectin receptors on immature thymocytes.

The Journal of cell biology ·Vol. 106 ·No. 6 ·1988-06-00 ·Pages 2183-90

Cardarelli PM, Crispe IN, Pierschbacher MD

Abstract

Fibronectin-adherent (FNR+) thymocytes are enriched for immature (CD4-8-) and large (CD4+8+) cells, and depleted of mature (CD4-8+ and CD4+8-) and nonmature small (CD4+8+) cells. Among purified CD4-8- thymocytes, cells with the surface marker J11d and the IL-2 receptor, which can give rise to all other thymocyte subsets, showed selective attachment to fibronectin. Analysis of FNR+ thymocytes showed that such cells are greatly enriched for cells in cycle. Additionally, FNR+ cells expressed low levels of T cell receptor. These results suggest a role for the fibronectin receptor during the early, proliferative phase of thymocyte differentiation. The data suggest that loss of the fibronectin receptor is a hallmark of cells that have become committed either to functional maturation or to programmed cell death.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte/analysis Cell Adhesion Cell Cycle Cell Differentiation Fibronectins/physiology Flow Cytometry Immunologic Techniques Mice Receptors, Antigen, T-Cell/metabolism Receptors, Antigen, T-Cell, alpha-beta Receptors, Fibronectin Receptors, Immunologic/physiology T-Lymphocytes/classification,physiology Thymus Gland/cytology
Chemicals
Antigens, Differentiation, T-Lymphocyte Fibronectins Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta Receptors, Fibronectin Receptors, Immunologic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cardarelli P M
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.
Crispe I N
Pierschbacher M D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.6.2183
Published
1988-06-00
Pages
2183-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115119
Subset
IM
Grants
NIAID NIH HHS · AI 19335 · United States
NCI NIH HHS · CA 30199 · United States
NCI NIH HHS · CA 38352 · United States
External Links
PubMed source DOI: 10.1083/jcb.106.6.2183 PMC Full Text View journal details
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