Home LiteratureArticle Details
PMID: 2951368 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proton-motive-force-dependent step in the pathway to lysis of Escherichia coli induced by bacteriophage phi X174 gene E product.

Journal of bacteriology ·Vol. 169 ·No. 4 ·1987-04-00 ·Pages 1750-2

Witte A, Lubitz W, Bakker EP

Abstract

We examined the cellular effects after the expression of the cloned lysis gene E of bacteriophage phi X174. Chloramphenicol prevented lysis only when added within the first minute of derepression of E synthesis, indicating that a time lag of several minutes exists between the synthesis of the E protein and the onset of cell lysis. Experiments with protonophores showed the existence of a subsequent step dependent on proton motive force at about 3 to 5 min before lysis.

MeSH Terms
Adenosine Triphosphate/metabolism Bacteriolysis/drug effects Bacteriophage phi X 174/genetics,physiology Chloramphenicol/pharmacology Escherichia coli/metabolism Genes, Viral Hydrogen-Ion Concentration Viral Proteins/genetics,physiology
Chemicals
Viral Proteins Chloramphenicol Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Witte A
Lubitz W
Bakker E P
References (12)
12 references, click to expand
  1. The use of several energy-coupling reactions in characterizing mutants of Escherichia coli K12 defective in oxidative phosphorylation.
    Eur J Biochem. 1976 JUL 1;66(2):257-68 PMID: 133025
  2. Overlapping genes in bacteriophage phiX174.
    Nature. 1976 Nov 4;264(5581):34-41 PMID: 1004533
  3. Autolysis of Escherichia coli.
    J Bacteriol. 1980 Apr;142(1):52-9 PMID: 6768717
  4. Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4669-73 PMID: 7001463
  5. Energy is required for maturation of exported proteins in Escherichia coli.
    Eur J Biochem. 1981 May 15;116(2):227-33 PMID: 7018904
  6. Lysis of Escherichia coli by cloned phi X174 gene E depends on its expression.
    J Gen Microbiol. 1985 May;131(5):1107-14 PMID: 3160821
  7. Lysis of Escherichia coli by induction of cloned phi X174 genes.
    Mol Gen Genet. 1982;185(3):493-7 PMID: 6285147
  8. Lytic action of cloned phi X174 gene E.
    J Virol. 1982 Dec;44(3):993-1002 PMID: 6294347
  9. Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.
    Gene. 1983 Apr;22(1):103-13 PMID: 6305768
  10. The requirement for energy during export of beta-lactamase in Escherichia coli is fulfilled by the total protonmotive force.
    EMBO J. 1984 Apr;3(4):895-900 PMID: 6327294
  11. Subcellular localization of lethal lysis proteins of bacteriophages lambda and phiX174.
    J Virol. 1985 Mar;53(3):1008-11 PMID: 3156254
  12. Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5396-400 PMID: 7029533
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.4.1750-1752.1987
Published
1987-04-00
Pages
1750-2
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212011
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.4.1750-1752.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2