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PMID: 2947238 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bacteriophage lambda cro mutations: effects on activity and intracellular degradation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 23 ·1986-12-00 ·Pages 8829-33

Pakula AA, Young VB, Sauer RT

Abstract

Following random mutagenesis of the bacteriophage lambda cro gene, we have isolated missense mutations that affect approximately half of the 66 residue positions of Cro. About two-thirds of the mutations change residues involved in the maintenance of Cro structure and stability. The corresponding mutant proteins are severely degraded in the cell but often have specific activities near that of wild-type Cro. The remaining mutations affect residues involved in DNA binding. These mutant proteins are present at moderately reduced intracellular levels, but their specific activities are much lower than that of wild type.

MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics DNA-Binding Proteins Mutation Promoter Regions, Genetic Protein Biosynthesis Protein Conformation Repressor Proteins/genetics,metabolism Structure-Activity Relationship Transcription Factors/genetics Viral Proteins Viral Regulatory and Accessory Proteins
Chemicals
DNA-Binding Proteins Repressor Proteins Transcription Factors Viral Proteins Viral Regulatory and Accessory Proteins phage repressor proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pakula A A
Young V B
Sauer R T
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.23.8829
Published
1986-12-00
Pages
8829-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387026
Subset
IM
Grants
NIAID NIH HHS · AI-16892 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.23.8829 PMC Full Text View journal details
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