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PMID: 2946649 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Calcium-stimulated myofibrillar ATPase activity correlates with shortening velocity of muscle fibres in Xenopus laevis.

The Histochemical journal ·Vol. 18 ·No. 9 ·1986-09-00 ·Pages 487-96

van der Laarse WJ, Diegenbach PC, Hemminga MA

Abstract

The iliofibularis muscle of Xenopus laevis is reported to contain five types of fibres which have different force-velocity relationships. Ten fibres of each type were selected on the basis of succinate dehydrogenase activity, cross-sectional area and location in the muscle, in order to assess the validity of the fibre type classification. Maximum calcium-stimulated myofibrillar ATPase activity (Vmax) and apparent Michaelis constant (Km) for ATP were determined for these 50 fibres from serial sections. The values obtained varied according to the type of fibre. Type 1 had the highest and type 5 the lowest values for Km and Vmax. In a separate experiment, single freeze-dried fibres were used to determine the relationship between their ATP content and apparent Km for ATP. There was a tendency for high ATP concentrations in fibres with high Km values. When myofibrillar ATPase activity was related to the maximum velocity of shortening of the five fibre types, a significant correlation was found. It is concluded that calcium-stimulated myofibrillar ATPase histochemistry allows an estimate of the maximum shortening velocity of muscle fibres from Xenopus laevis.

MeSH Terms

Adenosine Triphosphatases/analysis Analysis of Variance Animals Calcium/physiology Female Freeze Drying Histocytochemistry Kinetics Muscle Contraction Muscles/enzymology,physiology Succinate Dehydrogenase/analysis Xenopus laevis

Chemicals

Succinate Dehydrogenase Adenosine Triphosphatases Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

van der Laarse W J

Diegenbach P C

Hemminga M A

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23 references, click to expand

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Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01675616

Published

1986-09-00

Pages

487-96

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01675616

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Calcium-stimulated myofibrillar ATPase activity correlates with shortening velocity of muscle fibres in Xenopus laevis.

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