Depletion of suppressor T cells by 2'-deoxyguanosine abrogates tolerance in mice fed ovalbumin and permits the induction of intestinal delayed-type hypersensitivity.

Immunology ·Vol. 58 ·No. 2 ·1986-06-00 ·Pages 179-84

Mowat AM

Abstract

We have re-examined the role of suppressor T cells (Ts) in regulating immune responses to fed proteins by investigating the effect of 2'-deoxyguanosine (dGuo) on systemic and intestinal immunity in mice fed ovalbumin (OVA). Administration of dGuo for 10 days abrogated the suppression of systemic delayed-type hypersensitivity (DTH) and antibody responses normally found after feeding OVA, and also prevented the generation of OVA-specific Ts. In parallel, mice given dGuo and fed OVA developed sensitization to OVA in the gut-associated lymphoid tissues (GALT) after oral challenge with OVA and had increased intraepithelial lymphocyte (IEL) counts and crypt cell production rates (CCPR) in the jejunal mucosa, indicating the presence of a local DTH response. These findings confirm the importance of Ts in preventing hypersensitivity to dietary protein antigens and suggest that enteropathies associated with food hypersensitivity are due to a defect in Ts activity.

MeSH Terms

Animals Deoxyguanosine/pharmacology Dietary Proteins/immunology Female Hypersensitivity, Delayed/immunology Immune Tolerance Immunoglobulin G/biosynthesis Intestinal Mucosa/immunology Leukocyte Count Lymphocyte Depletion Mice Mice, Inbred BALB C Ovalbumin/immunology T-Lymphocytes, Regulatory/drug effects,immunology

Chemicals

Dietary Proteins Immunoglobulin G Ovalbumin Deoxyguanosine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Mowat A M

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28 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1986-06-00

Pages

179-84

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1452666

Subset

External Links

PubMed source PMC Full Text View journal details

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Depletion of suppressor T cells by 2'-deoxyguanosine abrogates tolerance in mice fed ovalbumin and permits the induction of intestinal delayed-type hypersensitivity.

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