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PMID: 2932273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of inhibitors of glycosylation and carbohydrate processing on invasion of malignant mouse MO4 cells in organ culture.

Clinical & experimental metastasis ·Vol. 3 ·No. 3 ·1985-00-00 ·Pages 197-207

Mareel MM, Dragonetti CH, Hooghe RJ, Bruyneel EA

Abstract

Inhibitors of glycosylation and carbohydrate processing were used to investigate the role of carbohydrates exposed at the cell surface in invasion. Malignant mouse MO4 cells were confronted with embryonic chick heart in organ culture, an assay shown to be relevant for a number of aspects of invasion in vivo. Tunicamycin (1.0 microgram/ml), 2-deoxy-D-glucose (100 mM), beta-OH-norvaline (1.0 mM), and Monensin (0.1 microgram/ml) reversibly inhibited the invasion of MO4 cells. At these concentrations the drugs also inhibited the growth of MO4 cells. 1-Deoxynojirimycin (10mM), swainsonine (0.4 microgram/ml), and Marcellomycin (0.1 microgram/ml) permitted invasion. Marcellomycin also reversibly inhibited the growth of MO4 cells. These results show that drugs known to interfere with the glycosylation or processing of carbohydrate chains of glycoproteins in different ways have different effects on the invasion of MO4 cells in vitro.

MeSH Terms
1-Deoxynojirimycin Alkaloids/pharmacology Animals Anthracyclines Antibiotics, Antineoplastic/pharmacology Carbohydrate Metabolism Cell Communication/drug effects Cell Division/drug effects Cell Membrane/drug effects,metabolism Cells, Cultured Chick Embryo Deoxyglucose/pharmacology Glucosamine/analogs & derivatives,pharmacology Glycoproteins/metabolism Heart Mice Monensin/pharmacology Naphthacenes/pharmacology Neoplasms Sucrase/antagonists & inhibitors Swainsonine Threonine/analogs & derivatives,pharmacology Tunicamycin/pharmacology
Chemicals
Alkaloids Anthracyclines Antibiotics, Antineoplastic Glycoproteins Naphthacenes Tunicamycin 1-Deoxynojirimycin 3-hydroxynorvaline Threonine marcellomycin Monensin Deoxyglucose Sucrase Glucosamine Swainsonine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mareel M M
Dragonetti C H
Hooghe R J
Bruyneel E A
References (36)
36 references, click to expand
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Article Info
Journal
Clinical & experimental metastasis
Abbr.
Clin Exp Metastasis
ISSN
0262-0898
DOI
10.1007/BF01786763
Published
1985-00-00
Pages
197-207
Language
English
Region
Netherlands
NLM ID
8409970
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01786763 View journal details
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