Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.

Nucleic acids research ·Vol. 17 ·No. 5 ·1989-03-11 ·Pages 2141

Ferre F, Garduno F

Abstract

暂无摘要

MeSH Terms

Cell Extracts/analysis Cell Line DNA-Directed DNA Polymerase Gene Amplification Humans Leukemia, Erythroblastic, Acute RNA, Messenger/isolation & purification Taq Polymerase Tissue Extracts/analysis

Chemicals

Cell Extracts RNA, Messenger Tissue Extracts Taq Polymerase DNA-Directed DNA Polymerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ferre F

Cytometrics Inc., Division of Specialty Laboratories Inc., San Diego, CA 92121.

Garduno F

References (1)

1 references, click to expand

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/17.5.2141

Published

1989-03-11

Pages

2141

Language

English

Region

England

NLM ID

0411011

PMCID

PMC317567

Subset

External Links

PubMed source DOI: 10.1093/nar/17.5.2141 PMC Full Text View journal details

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Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.

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