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PMID: 2927394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electron microscopy reveals that transcription factor TFIIIA bends 5S DNA.

Molecular and cellular biology ·Vol. 9 ·No. 1 ·1989-01-00 ·Pages 336-41

Bazett-Jones DP, Brown ML

Abstract

We have used a high-resolution analytical electron microscopic technique, electron spectroscopic imaging, to study the in vitro interaction between the transcription factor IIIA (TFIIIA) and 5S ribosomal gene DNA. The images and analytical measurements support our proposal that the helix axis is bent by the protein into a hairpin-shaped configuration.

MeSH Terms
Animals Cattle DNA, Ribosomal/ultrastructure DNA-Binding Proteins/metabolism Genes Microscopy, Electron Nucleic Acid Conformation Ovum/cytology RNA, Ribosomal/ultrastructure RNA, Ribosomal, 5S/ultrastructure Transcription Factor TFIIIA Transcription Factors/ultrastructure Xenopus laevis
Chemicals
DNA, Ribosomal DNA-Binding Proteins RNA, Ribosomal RNA, Ribosomal, 5S Transcription Factor TFIIIA Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bazett-Jones D P
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Brown M L
References (19)
19 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.1.336-341.1989
Published
1989-01-00
Pages
336-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362179
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.1.336-341.1989 PMC Full Text View journal details
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