Home LiteratureArticle Details
PMID: 291994 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cholesterol homeostasis in the rat with a portacaval anastomosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4654-7

Proia A, McNamara DJ, Edwards KD, Ahrens EH

Abstract

Studies were undertaken to determine the effect of portacaval anastomosis on cholesterol homeostasis in rats fed sucrose/lard under conditions of normal body growth. Four to 6 weeks after portacaval shunt surgery, we found decreases in plasma cholesterol and triglyceride concentrations, total liver weight, and hepatic microsomal protein concentration. Measurements oof hepatic 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) activity showed decreases in specific activity and total liver activity in portacaval shunt rats, but the enzyme diurnal rhythm remained. Decreased reductase activity in shunted rats was not due to an altered Km for D-HMG-COA, nor was an enzyme inhibitor found in the livers of the portacaval shunt animals. Sterol balance measurements in rats with shunts showed a 22% decrease in whole body cholesterol synthesis rate compared to controls. These metabolic studies, coupled with postmortem data, showed diminished bile acid synthesis, unchanged fecal neutral steroid excretion, and decreased net tissue accumulation of cholesterol during growth. The decreased whole body cholesterol synthesis rate ultimately led to a diminished total carcass cholesterol concentration in the rats with shunts.

MeSH Terms
Animals Cholesterol/metabolism Dietary Carbohydrates/metabolism Growth Homeostasis Hydroxymethylglutaryl CoA Reductases/metabolism Hyperlipoproteinemia Type II/surgery Kinetics Liver/enzymology Portacaval Shunt, Surgical Rats
Chemicals
Dietary Carbohydrates Cholesterol Hydroxymethylglutaryl CoA Reductases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Proia A
McNamara D J
Edwards K D
Ahrens E H
References (28)
28 references, click to expand
  1. QUANTITATIVE ISOLATION AND GAS--LIQUID CHROMATOGRAPHIC ANALYSIS OF TOTAL FECAL BILE ACIDS.
    J Lipid Res. 1965 Jul;6:397-410 PMID: 14336211
  2. QUANTITATIVE ISOLATION AND GAS--LIQUID CHROMATOGRAPHIC ANALYSIS OF TOTAL DIETARY AND FECAL NEUTRAL STEROIDS.
    J Lipid Res. 1965 Jul;6:411-24 PMID: 14340676
  3. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  4. Portacaval shunt and hyperlipidemia.
    Arch Surg. 1978 Jan;113(1):71-4 PMID: 202223
  5. Effect of portacaval anastomosis on hepatic HMG-CoA-reductase activity in normal rats.
    FEBS Lett. 1977 Mar 15;75(1):101-4 PMID: 192584
  6. Regulation of sterol synthesis in 16 tissues of rat. I. Effect of diurnal light cycling, fasting, stress, manipulation of enterohepatic circulation, and administration of chylomicrons and triton.
    J Biol Chem. 1977 Jun 10;252(11):3646-51 PMID: 558975
  7. Plasma lipids and hepatic lipid synthesis following portacaval shunt in the rat.
    S Afr Med J. 1978 Jun 17;53(25):1024-6 PMID: 694673
  8. Hepatic 3-hydroxy-3-methylglutaryl coenzyme-a reductase of rats fed semipurified and stock diets.
    J Nutr. 1977 Mar;107(3):453-7 PMID: 845681
  9. Lowering of blood pressure, plasma renin substrate, cholesterol and triglyceride by portacaval anastomosis in rats fed on a 60% sucrose/5% lard diet.
    Clin Sci Mol Med Suppl. 1976 Dec;3:145s-146s PMID: 1071592
  10. Plasma renin substrate and blood pressure after protacaval anastomosis.
    Med J Aust. 1976 Oct 9;2(15):581 PMID: 994972
  11. Determination of the michaelis-menten constant for beta-hydroxy-beta-methylglutaryl coenzyme A reductase. Demonstration of a substrate affinity 10-fold greater than previously reported.
    J Biol Chem. 1976 Sep 25;251(18):5820-3 PMID: 965392
  12. Micro assay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts.
    Biochim Biophys Acta. 1974 Dec 29;370(2):369-77 PMID: 4441486
  13. The intestine is the major site of cholesterol synthesis in the rat.
    Nat New Biol. 1973 Mar 14;242(115):61-2 PMID: 4512013
  14. Portacaval shunt in hyperlipoproteinaemia.
    Lancet. 1973 Oct 27;2(7835):940-4 PMID: 4126562
  15. Hepatic encephalopathy and light and electron micrographic changes of the baboon liver after portal diversion.
    Ann Surg. 1976 Aug;184(2):155-61 PMID: 821406
  16. Effect of portacaval anastomosis on the activities of hepatic enzymes related to cholesterol and bile acid metabolism in rats.
    Biochim Biophys Acta. 1976 Aug 23;441(2):308-15 PMID: 8128
  17. Cholesterol metabolism following portacaval shunt in the pig.
    Atherosclerosis. 1976 Jul-Aug;24(1-2):141-8 PMID: 182182
  18. The effect of portacaval shunt on plasma lipids and tissue cholesterol synthesis in the dog.
    Surgery. 1976 Jul;80(1):54-60 PMID: 179161
  19. Reduction in cholesterol and low density lipoprotein synthesis after portacaval shunt surgery in a patient with homozygous familial hypercholesterolemia.
    J Clin Invest. 1975 Dec;56(6):1420-30 PMID: 172531
  20. The role of the intestinal mucosa in cholesterol metabolism: its relation to plasma and luminal cholesterol.
    Exp Mol Pathol. 1974 Aug;21(1):138-53 PMID: 4423939
  21. The Eck fistula rat: definition of an experimental model.
    Eur J Clin Invest. 1972 Nov;2(6):390-7 PMID: 4676320
  22. Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase. Developmental pattern.
    J Biol Chem. 1972 Sep 25;247(18):5805-10 PMID: 5057084
  23. Regulation of HMG-CoA reductase.
    Adv Lipid Res. 1976;14:1-74 PMID: 769497
  24. Blood pressure, cholesterol and triglyceride lowering effects of portacaval anastomosis. Efficacy in the rat, associated with hepatic weight reduction and cardiac and renal hypertrophy.
    Med J Aust. 1976 Mar 27;1(13):450-1 PMID: 1272140
  25. Measurement of squalene in human tissues and plasma: validation and application.
    J Lipid Res. 1976 Jan;17(1):38-45 PMID: 1255019
  26. Newly synthesized hepatic cholesterol as precursor for cholesterol and bile acids in rat bile.
    Scand J Gastroenterol. 1976;11(4):427-32 PMID: 935805
  27. An improved method for the isolation, quantitation, and identification of bile acids in rats feces.
    Anal Biochem. 1975 Apr;64(2):567-77 PMID: 1130667
  28. Experimental evaluation of liver atrophy and portacaval shunt.
    Surg Gynecol Obstet. 1967 Dec;125(6):1253-8 PMID: 6065262
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4654
Published
1979-09-00
Pages
4654-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411638
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4654 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2