Home LiteratureArticle Details
PMID: 291927 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis and processing of human chorionic gonadotropin subunits in cultured choriocarcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 10 ·1979-10-00 ·Pages 5143-7

Ruddon RW, Hanson CA, Addison NJ

Abstract

Pulse and pulse-chase experiments have identified the presence of partially glycosylated precursors of the alpha and beta subunits of human chorionic gonadotropin (hCG) in cultured JAR choriocarcinoma cells. The alpha subunit precursor has an apparent molecular weight (by sodium dodecyl sulfate/polyacrylamide gel electrophoresis) of 18,000 (compared to 22,000 for fully processed alpha subunit); the beta subunit precursor has an apparent molecular weight of 24,000 (fully processed, 34,000). Both of these precursors appear to have an intracellular half-life of at least 1 hr and to contain the mannose core but not the terminal carbohydrate sequences. Fully processed alpha and beta subunits do not accumulate intracellularly, indicating that further processing of the precursors is followed by rapid secretion.

MeSH Terms
Carbohydrate Sequence Cell Line Choriocarcinoma/metabolism Chorionic Gonadotropin/biosynthesis Female Humans Macromolecular Substances Molecular Weight Pregnancy Tunicamycin/pharmacology
Chemicals
Chorionic Gonadotropin Macromolecular Substances Tunicamycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ruddon R W
Hanson C A
Addison N J
References (26)
26 references, click to expand
  1. Heterogeneity of the human chorionic gonadotropin alpha-subunit secreted by cultured choriocarcinoma (JEG) cells.
    J Clin Endocrinol Metab. 1979 Jan;48(1):85-91 PMID: 217890
  2. Glycosylation of a membrane protein is restricted to the growing polypeptide chain but is not necessary for insertion as a transmembrane protein.
    Cell. 1978 Dec;15(4):1447-54 PMID: 215329
  3. Processing of high mannose oligosaccharides to form complex type oligosaccharides on the newly synthesized polypeptides of the vesicular stomatitis virus G protein and the IgG heavy chain.
    J Biol Chem. 1978 Feb 10;253(3):716-22 PMID: 202596
  4. Oligosaccharide chains are trimmed during synthesis of the envelope glycoprotein of vesicular stomatitis virus.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):754-8 PMID: 204932
  5. Effects of sodium butyrate on synthesis of human chorionic gonadotrophin in trophoblastic and non-trophoblastic tumours.
    Nature. 1977 Aug 11;268(5620):543-4 PMID: 196218
  6. Immunologic and physical characterization of human chorionic gonadotropin and its subunits in cultures of human malignant trophoblast.
    J Clin Endocrinol Metab. 1977 Jun;44(6):1154-62 PMID: 194911
  7. Coupled cell-free synthesis, segregation, and core glycosylation of a secretory protein.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2338-42 PMID: 276877
  8. mRNA-dependent synthesis of a glycosylated subunit of human chorionic gonadotropin in cell-free extracts derived from ascites tumor cells.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1768-72 PMID: 273908
  9. Processing of placental peptide hormones synthesized in lysates containing membranes derived from tunicamycin-treated ascites tumor cells.
    J Biol Chem. 1978 Oct 25;253(20):7117-9 PMID: 568137
  10. Structure of the murine leukemia virus envelope glycoprotein precursor.
    J Virol. 1979 Feb;29(2):735-43 PMID: 430608
  11. Isolation and characterization of N- and O- glycosidic carbohydrate units of human chorionic gonadotropin.
    Biochem Biophys Res Commun. 1978 Oct 16;84(3):667-76 PMID: 718709
  12. Ectopic production of human chorionic gonadotropin in malignant tumors.
    Cancer. 1978 Nov;42(5):2328-33 PMID: 719611
  13. Precursor-product relationships in the biosynthesis and secretion of thyrotropin and its subunits by mouse thyrotropic tumor cells.
    FEBS Lett. 1978 Aug 15;92(2):303-7 PMID: 700102
  14. Identification of mRNAs encoding the alpha and beta subunits of human choriogonadotropin.
    J Biol Chem. 1978 Oct 10;253(19):7109-14 PMID: 690142
  15. Evidence for the participation of saccharide-lipids in the synthesis of the oligosaccharide chain of ovalbumin.
    J Biol Chem. 1977 Feb 10;252(3):1007-13 PMID: 838693
  16. Tunicamycin, a new antibiotic. I. Isolation and characterization of tunicamycin.
    J Antibiot (Tokyo). 1971 Apr;24(4):215-23 PMID: 5572750
  17. Endo-beta-N-acetylglucosaminidase acting on carbohydrate moieties of glycoproteins. Purification and properties of the enzyme from Diplococcus pneumoniae.
    J Biol Chem. 1974 Aug 10;249(15):4897-904 PMID: 4152561
  18. The release of intact oligosaccharides from specific glycoproteins by endo-beta-N-acetylglucosaminidase H.
    J Biol Chem. 1974 Feb 10;249(3):818-24 PMID: 4204553
  19. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1419-22 PMID: 4514312
  20. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  21. Ectopic production of the isolated alpha subunit of the glycoprotein hormones. A quantitative marker in certain cases of cancer.
    N Engl J Med. 1974 Jun 27;290(26):1441-7 PMID: 4831760
  22. Ectopic production of the isolated beta subunit of human chorionic gonadotropin.
    J Clin Invest. 1973 Dec;52(12):3135-42 PMID: 4750453
  23. Gonadotropins and their subunits: basic and clinical studies.
    Recent Prog Horm Res. 1976;32:289-331 PMID: 785557
  24. Pre-proparathyroid hormone. Evidence for an early biosynthetic precursor of proparathyroid hormone.
    J Biol Chem. 1976 Jul 10;251(13):3893-9 PMID: 932012
  25. Differences between purified ectopic and normal alpha subnits of human glycoprotein hormones.
    J Clin Invest. 1975 Oct;56(4):1043-52 PMID: 1159086
  26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.10.5143
Published
1979-10-00
Pages
5143-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC413096
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.10.5143 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2