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PMID: 2919174 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fourier analysis of cell motility: correlation of motility with metastatic potential.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 4 ·1989-02-00 ·Pages 1254-8

Partin AW, Schoeniger JS, Mohler JL, Coffey DS

Abstract

We report the development of a computerized, mathematical system for quantitating the various types of cell motility. This Fourier analysis method simultaneously quantifies for individual cells (i) temporal changes in cell shape represented by cell ruffling, undulation, and pseudopodal extension, (ii) cell translation, and (iii) average cell size and shape. This spatial-temporal Fourier analysis was tested on a series of well-characterized animal tumor cell lines of rat prostatic cancer to study in a quantitative manner the correlation of cell motility with increasing in vivo metastatic potential. Fourier motility coefficients measuring pseudopodal extension correlated best with metastatic potential in the cell lines studied. This study demonstrated that Fourier analysis provides quantitative measurement of cell motility that may be applied to the study of biological processes. This analysis should aid in the study of the motility of individual cells in various areas of cellular and tumor biology.

MeSH Terms
Adenocarcinoma/pathology,physiopathology Animals Cell Line Cell Membrane/physiology,ultrastructure Cell Movement Fourier Analysis Male Models, Theoretical Neoplasm Metastasis/pathology,physiopathology Prostatic Neoplasms/pathology,physiopathology Rats
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Partin A W
Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Schoeniger J S
Mohler J L
Coffey D S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.4.1254
Published
1989-02-00
Pages
1254-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286666
Subset
IM
Grants
NIGMS NIH HHS · 5T32 GM07309 · United States
NCI NIH HHS · CA 15416 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.4.1254 PMC Full Text View journal details
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