Home LiteratureArticle Details

PMID: 2916329 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends.

Virology ·Vol. 168 ·No. 2 ·1989-02-00 ·Pages 363-9

Rohozinski J, Girton LE, Van Etten JL

Abstract

Pulsed field electrophoresis established that Chlorella viruses contain linear, nonpermuted, 330- to 380-kb dsDNA genomes. Terminal DNA restriction fragments of one virus, PBCV-1, were identified by Bal31 exonuclease digestion; the termini probably contain covalently closed hairpin ends. The end fragments cross-hybridize indicating terminal repetition; the region of repetition extends no more than 2.5 kb from the ends.

MeSH Terms

Chlorella DNA, Viral/analysis,genetics Genes, Viral Nucleic Acid Conformation Nucleic Acid Hybridization Restriction Mapping Viruses/genetics

Chemicals

DNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rohozinski J

Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.

Girton L E

Van Etten J L

Article Info

Journal

Virology

Abbr.

Virology

ISSN

0042-6822

DOI

10.1016/0042-6822(89)90277-8

Published

1989-02-00

Pages

363-9

Language

English

Region

United States

NLM ID

0110674

Subset

Grants

NIGMS NIH HHS · GM32441 · United States

External Links

PubMed source DOI: 10.1016/0042-6822(89)90277-8 View journal details

Analysis Services

Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends.

Contact

WeChat Official Account