Abstract
Addition of ATP to a highly purified fraction of rat liver nuclei incubated with submicromolar concentrations of Ca2+ and trace amounts of 45Ca2+ resulted in the rapid accumulation of 45Ca2+ in the nuclei. This was associated with an increase in intranuclear free Ca2+ concentration as measured with the fluorescent dye 1-[2-(5-carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-a mino-5'- methylphenoxy)ethane-N,N,N',N'-tetraacetic acid (fura-2). Inhibitors of microsomal and mitochondrial Ca2+ translocases had no effect on nuclear Ca2+ sequestration, indicating that it was distinct from previously known intracellular Ca2+-transporting systems. Ca2+ uptake and the associated increase in intranuclear free Ca2+ concentration were prevented by calmidazolium, a potent calmodulin antagonist. Partial characterization of the ATP-stimulated nuclear Ca2+ uptake showed that maximal rates of Ca2+ uptake and increase in intranuclear free Ca2+ level occurred at concentrations of Ca2+ normally present in the cytosol of mammalian cells. Together, these results show that a distinct, ATP- and calmodulin-dependent Ca2+ uptake system exists in liver nuclei. This system may play an important role in the regulation of intranuclear Ca2+-dependent processes.
MeSH Terms
Adenosine Triphosphate/pharmacology
Animals
Biological Transport, Active
Calcium/metabolism
Calmodulin/antagonists & inhibitors,metabolism
Cell Nucleus/metabolism
Imidazoles/pharmacology
Liver/metabolism,ultrastructure
Male
Microscopy, Fluorescence
Rats
Rats, Inbred Strains
Chemicals
Calmodulin
Imidazoles
calmidazolium
Adenosine Triphosphate
Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nicotera P
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.
McConkey D J
Jones D P
Orrenius S
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