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PMID: 2911467 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A 12 megabase restriction map at the cystic fibrosis locus.

Nucleic acids research ·Vol. 17 ·No. 1 ·1989-01-11 ·Pages 271-84

Fulton TR, Bowcock AM, Smith DR, Daneshvar L, Green P, Cavalli-Sforza LL, Donis-Keller H

Abstract

We have constructed a physical map of the chromosomal region containing the cystic fibrosis locus using seven DNA markers and pulsed-field gel electrophoresis methods. The map includes cleavage sites for 8 rare-cutting restriction enzymes and spans over 12 megabases (Mb) of DNA, with one unlinked probe covering an additional 5 Mb. To our knowledge, this is the largest segment of human DNA which has been restriction-mapped to date. We can identify thirteen putative HTF islands spaced at intervals of 0.3-3.2 Mb. The region between loci D7S8 and MET, where the CF gene lies, includes 1.4-1.9 Mb of DNA.

MeSH Terms
Blotting, Southern Cell Line Chromosome Mapping Chromosomes, Human, Pair 7 Cystic Fibrosis/genetics DNA/genetics,isolation & purification Genetic Linkage Genetic Markers/analysis Humans Molecular Weight Nucleic Acid Hybridization Restriction Mapping
Chemicals
Genetic Markers DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fulton T R
Department of Human Genetics, Collaborative Research Inc., Bedford, MA.
Bowcock A M
Smith D R
Daneshvar L
Green P
Cavalli-Sforza L L
Donis-Keller H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.1.271
Published
1989-01-11
Pages
271-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331550
Subset
IM
Grants
PHS HHS · 28428 · United States
PHS HHS · 37656 · United States
External Links
PubMed source DOI: 10.1093/nar/17.1.271 PMC Full Text View journal details
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