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PMID: 290995 Published · ppublish English Journal Article

Nucleoside triphosphate regeneration decreases the frequency of translation errors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3174-8

Jelenc PC, Kurland CG

Abstract

The addition of naturally occurring polyamines and inorganic ions to an in vitro protein-synthesizing system improved the extent and fidelity of translation. In such an optimized system, regeneration of the nucleoside triphosphates with phosphoenolpyruvate and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) reduced further the missense error frequency to the in vivo level as well as enhanced the extent of translation. The effect of nucleoside triphosphate regeneration was shown to be due primarily to the increase in the ratio of adenosine and guanosine triphosphates to their hydrolysis products and only marginally due to the increase in the absolute concentrations of the nucleoside triphosphates.

MeSH Terms
Adenosine Monophosphate/administration & dosage Adenosine Triphosphate/administration & dosage,metabolism Guanosine Diphosphate/administration & dosage Guanosine Triphosphate/administration & dosage,metabolism Poly U Protein Biosynthesis/drug effects
Chemicals
Guanosine Diphosphate Poly U Adenosine Monophosphate Guanosine Triphosphate Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jelenc P C
Kurland C G
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3174
Published
1979-07-00
Pages
3174-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383786
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3174 PMC Full Text View journal details
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