2 micrometer covalently closed non-mitochondrial circular DNA in the petite-negative yeast Schizosaccharomyces pombe.

Molecular & general genetics : MGG ·Vol. 172 ·No. 2 ·1979-05-04 ·Pages 165-9

Del Giudice L, Wolf K, Sassone-Corsi P, Mazza A

Abstract

A population of small covalently closed non-mitochondrial circular DNA molecules was isolated from the petite-negative yeast Schizosaccharomyces pombe. The mean length of these molecules, possessing the same density as nuclear DNA (1.695 g/cm3) is 1.95 +/- 0.18 micrometer. The presence of these minicircles in crude mitochondrial preparations indicates their tight association with mitochondrial particles. Their disappearance after DNase treatment of mitochondria demonstrates their extramitochondrial location.

MeSH Terms

Ascomycota/genetics Cell Fractionation Centrifugation, Density Gradient DNA, Circular/isolation & purification Microscopy, Electron Mitochondria Nucleic Acid Conformation Phenotype Schizosaccharomyces/genetics

Chemicals

DNA, Circular

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Del Giudice L

Wolf K

Sassone-Corsi P

Mazza A

References (31)

31 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268278

Published

1979-05-04

Pages

165-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268278

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2 micrometer covalently closed non-mitochondrial circular DNA in the petite-negative yeast Schizosaccharomyces pombe.

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