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PMID: 2896353 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and sequence of a cDNA clone encoding the 31-kDa subunit of bovine kidney vacuolar H+-ATPase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 9 ·1988-05-00 ·Pages 3004-8

Hirsch S, Strauss A, Masood K, Lee S, Sukhatme V, Gluck S

Abstract

We have isolated a cDNA encoding the 31-kDa subunit of the bovine kidney vacuolar H+-ATPase. The composite sequence contains 1219 base pairs, which includes the entire 678-base-pair coding region. A lysine-rich sequence previously found in the Na+, K+-ATPase alpha subunit and the H+,K+-ATPase was identified in the 31-kDa subunit. An RNA blot and an immunoblot demonstrated variable 31-kDa subunit expression and immunoreactivity in different tissues; the highest levels were observed in kidney medulla and brain with both types of analysis. The isolation of a cDNA for the 31-kDa subunit is an important step in understanding this subunit's role in the function and regulation of the vacuolar H+-ATPase.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle DNA/analysis,isolation & purification Kidney/enzymology Molecular Sequence Data Molecular Weight Proton-Translocating ATPases/genetics
Chemicals
DNA Proton-Translocating ATPases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hirsch S
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Strauss A
Masood K
Lee S
Sukhatme V
Gluck S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.9.3004
Published
1988-05-00
Pages
3004-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280131
Subset
IM
Grants
NIDDK NIH HHS · DK38848 · United States
Databases
GENBANK
J03244
External Links
PubMed source DOI: 10.1073/pnas.85.9.3004 PMC Full Text View journal details
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