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PMID: 2895638 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The beta 2-adrenergic receptors of human epidermoid carcinoma cells bear two different types of oligosaccharides which influence expression on the cell surface.

The Biochemical journal ·Vol. 250 ·No. 1 ·1988-02-15 ·Pages 133-43

Cervantes-Olivier P, Delavier-Klutchko C, Durieu-Trautmann O, Kaveri S, Desmandril M, Strosberg AD

Abstract

The beta 2-adrenergic receptors of the human epidermoid carcinoma A431 cells reside on two polypeptide chains revealed by photoaffinity labelling with [125I]iodocyanopindolol-diazirine. These proteins correspond to two distinct populations of N-asparagine-linked glycoproteins: the 55-52 kDa molecules are associated with complex carbohydrate chain(s), the 65-63 kDa component with polymannosidic carbohydrate chain(s). Both types of receptors are present in preconfluent cells, but only the polymannosidic type is found in the postconfluent cells. Moreover, complex chains appear to be associated with the receptors with the highest affinity for (-)-isoproterenol and polymannosidic chains with the receptors with the lowest affinity for this agonist. the carbohydrate moiety of the beta-adrenergic receptor is involved in the expression and function of the beta 2-adrenergic receptors at the surface of the A431 cells, since tunicamycin and monensin, complete and partial inhibitors of glycosylation respectively, diminish the number of binding sites at the cell surface and increase the total number of sites in the cell. In these conditions a diminution of cyclic AMP accumulation is also observed.

MeSH Terms
Adrenergic beta-Antagonists/pharmacology Binding Sites Carcinoma, Squamous Cell/metabolism Cell Line Cell Membrane/metabolism Chromatography, Affinity Cyclic AMP/metabolism Glycosylation Humans Isoproterenol/pharmacology Monensin/pharmacology Oligosaccharides/analysis Propanolamines/pharmacology Receptors, Adrenergic, beta/drug effects,metabolism Tunicamycin/pharmacology
Chemicals
Adrenergic beta-Antagonists Oligosaccharides Propanolamines Receptors, Adrenergic, beta Tunicamycin Monensin Cyclic AMP Isoproterenol CGP 12177
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cervantes-Olivier P
Department of Biotechnology, Institut Pasteur, C.N.R.S., Paris, France.
Delavier-Klutchko C
Durieu-Trautmann O
Kaveri S
Desmandril M
Strosberg A D
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2500133
Published
1988-02-15
Pages
133-43
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148825
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2500133 PMC Full Text View journal details
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