Gaucher's disease. Factors affecting the 4-methylum-belliferyl-beta-D-glucosidase activity of cultured skin fibroblasts.

Clinical genetics ·Vol. 14 ·No. 2 ·1978-08-00 ·Pages 77-9

Butterworth J, Broadhead DM

Abstract

Phosphate-buffered saline was found to inhibit acid beta-glucosidase activity of cultured cells. This effect was attributable to both the pH and the chloride content of the phosphate-buffered saline. Washing the cells in sucrose (0.25M) to remove residual phosphate-buffered saline effectively eliminated this inhibition.

MeSH Terms

Buffers Cells, Cultured Chlorides/pharmacology Fibroblasts/enzymology Gaucher Disease/enzymology Glucosidases/metabolism Humans Hydrogen-Ion Concentration Phosphates Skin/enzymology Sodium Chloride/pharmacology Sucrose/pharmacology beta-Glucosidase/metabolism

Chemicals

Buffers Chlorides Phosphates Sodium Chloride Sucrose Glucosidases beta-Glucosidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Butterworth J

Broadhead D M

Article Info

Journal

Clinical genetics

Abbr.

Clin Genet

ISSN

0009-9163

DOI

10.1111/j.1399-0004.1978.tb02109.x

Published

1978-08-00

Pages

77-9

Language

English

Region

Denmark

NLM ID

0253664

Subset

External Links

PubMed source DOI: 10.1111/j.1399-0004.1978.tb02109.x View journal details

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Gaucher's disease. Factors affecting the 4-methylum-belliferyl-beta-D-glucosidase activity of cultured skin fibroblasts.

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