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PMID: 2885843 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Aberrant transmembrane signal transduction in Dictyostelium cells expressing a mutated ras gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 14 ·1987-07-00 ·Pages 4905-9

Van Haastert PJ, Kesbeke F, Reymond CD, Firtel RA, Luderus E, Van Driel R

Abstract

Dictyostelium discoideum cells contain a single ras gene (Dd-ras) that is highly homologous to mammalian ras genes. Cell transformation with a vector carrying a ras gene with a (glycine----threonine) missense mutation at position 12 causes an altered morphogenesis. Extracellular cAMP signals regulate morphogenesis and induce chemotaxis and the activation and subsequent desensitization of adenylate and guanylate cyclase. cAMP signal transduction was investigated in Dd-ras-transformed cells. Transformants that overexpress the mutated Dd-ras-Thr12 gene show normal activation and desensitization of adenylate cyclase and normal activation of guanylate cyclase. However, cAMP induces a stronger desensitization of guanylate cyclase stimulation in the Dd-ras-Thr12 transformant than in transformants overexpressing the Dd-ras-Gly12 wild-type gene or in untransformed cells. This effect was correlated with a reduced chemotactic sensitivity of the transformant expressing the mutated Dd-ras-Thr12 gene.

MeSH Terms
Adenylyl Cyclases/metabolism Chemotaxis Cyclic AMP/physiology Cyclic GMP/physiology Dictyostelium/genetics,physiology Enzyme Activation Fungal Proteins/genetics,physiology Guanylate Cyclase/metabolism Morphogenesis Oncogenes Transformation, Genetic ras Proteins
Chemicals
Fungal Proteins ras protein, Dictyostelium Cyclic AMP ras Proteins Adenylyl Cyclases Guanylate Cyclase Cyclic GMP
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Van Haastert P J
Kesbeke F
Reymond C D
Firtel R A
Luderus E
Van Driel R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.14.4905
Published
1987-07-00
Pages
4905-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305215
Subset
IM
Grants
NIGMS NIH HHS · GM 37830 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.14.4905 PMC Full Text View journal details
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