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PMID: 2883125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the S-1 catalytic subunit of pertussis toxin in Escherichia coli.

Infection and immunity ·Vol. 55 ·No. 5 ·1987-05-00 ·Pages 1321-3

Barbieri JT, Rappuoli R, Collier RJ

Abstract

The S-1 subunit of pertussis toxin was expressed as a fusion protein in a strain of Escherichia coli deficient in protein degradation. The fusion protein reacted with anti-pertussis toxin antibody, and, like authentic pertussis toxin, it ADP-ribosylated a 41,000-molecular-weight membrane protein from human erythrocytes.

MeSH Terms

Bordetella pertussis/genetics Cloning, Molecular Escherichia coli/genetics,metabolism Genes, Bacterial Pertussis Toxin Recombinant Fusion Proteins/biosynthesis,genetics Virulence Factors, Bordetella/genetics

Chemicals

Recombinant Fusion Proteins Virulence Factors, Bordetella Pertussis Toxin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Barbieri J T

Rappuoli R

Collier R J

References (10)

10 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.55.5.1321-1323.1987

Published

1987-05-00

Pages

1321-3

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC260508

Subset

Grants

NIAID NIH HHS · AI-22021 · United States

NCI NIH HHS · CA-39217 · United States

External Links

PubMed source DOI: 10.1128/iai.55.5.1321-1323.1987 PMC Full Text View journal details

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Expression of the S-1 catalytic subunit of pertussis toxin in Escherichia coli.

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