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PMID: 2879771 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning of nit-2, a regulatory gene required for nitrogen metabolite repression in Neurospora crassa.

Gene ·Vol. 46 ·No. 2-3 ·1986-00-00 ·Pages 291-5

Stewart V, Vollmer SJ

Abstract

We used an efficient sib-selection procedure to isolate a cosmid clone that complemented a mutated nit-2 gene of Neurospora crassa. Restriction fragment length polymorphism mapping indicated that the cosmid DNA insert was derived from linkage group IL, between 5S rDNA locus 12 and mt, the region of the N. crassa genome that contains nit-2. We conclude that the cosmid carries the nit-2 gene.

MeSH Terms

Chromosome Mapping Cloning, Molecular Cosmids Genes, Fungal Genes, Regulator Mutation Neurospora/genetics Neurospora crassa/genetics,metabolism Nitrogen/metabolism Polymorphism, Restriction Fragment Length

Chemicals

Nitrogen

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stewart V

Vollmer S J

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(86)90414-2

Published

1986-00-00

Pages

291-5

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Grants

NIGMS NIH HHS · GM 08736 · United States

External Links

PubMed source DOI: 10.1016/0378-1119(86)90414-2 View journal details

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Molecular cloning of nit-2, a regulatory gene required for nitrogen metabolite repression in Neurospora crassa.

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