Bacterial DNA synthesized under phage control in a DNA-defective Salmonella-mutant and packaged into a special fraction of transducing particles of phage P22.

Molecular & general genetics : MGG ·Vol. 171 ·No. 3 ·1979-03-27 ·Pages 301-6

Backhaus H, Schmieger H

Abstract

Lysates of P22 contain a small fraction of transducing particles with bacterial DNA replicated semiconservatively after the time of infection. It was demonstrated that the presence and relative amount of this class of transducing particles was unchanged, if infection of Salmonella occured under a condition nonpermissive for bacterial DNA replication. Analysis of particles with DNA fragments derived from different regions of the Salmonella chromosome indicated that the replication of the bacterial DNA carried by these transducing particles was not initiated specifically at the normal origin for bacterial chromosome replication.

MeSH Terms

DNA Replication DNA, Bacterial/biosynthesis DNA, Viral/genetics Hot Temperature Mutation Salmonella/genetics Salmonella Phages/genetics Time Factors Transduction, Genetic

Chemicals

DNA, Bacterial DNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Backhaus H

Schmieger H

References (23)

23 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267585

Published

1979-03-27

Pages

301-6

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267585

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Bacterial DNA synthesized under phage control in a DNA-defective Salmonella-mutant and packaged into a special fraction of transducing particles of phage P22.

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