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PMID: 2863165 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunocytochemical study of PC12 cells grafted to the brain of immature rats.

Experimental brain research ·Vol. 59 ·No. 3 ·1985-00-00 ·Pages 615-24

Jaeger CB

Abstract

Aggregated PC12 cells were transplanted to the brain of infant Sprague Dawley (SD) rats and grown for up to two months. Histochemical and immunocytochemical methods served to identify and characterize the grafted PC12 cells. It was found that PC12 cells persist and continue to proliferate within the brain of SD rats whereas it has previously been reported that grafts placed into peripheral sites do not proliferate. Dispersed and aggregated PC12 cell grafts contained immunoreactivity to tyrosine hydroxylase and aromatic amino acid decarboxylase, but failed to express phenylethanolamine n-methyl-transferase. Acetylcholinesterase activity was below histochemical detection levels in grafts of all ages. In aggregate grafts most PC12 cells were round but some small clusters of PC12 cells formed short processes. Process-forming PC12 cells were most prominent when positioned adjacent to deep layers of the cerebral cortex in one month old grafts. Host-derived capillaries and astroglia became incorporated into the PC12 aggregate grafts at successive stages. The blood brain barrier of the grafts was incomplete. This study indicates that grafted PC12 cells have some potential to restore function in brains that exhibit an endogenous dopamine deficiency, providing that mitosis in PC12 cells can be inhibited.

MeSH Terms
Acetylcholinesterase/analysis Adrenal Gland Neoplasms/metabolism,physiopathology Animals Animals, Newborn Brain/cytology,physiology Brain Chemistry Catecholamines/metabolism Cell Line Graft Survival Immunoenzyme Techniques Neoplasm Transplantation Neuroglia/metabolism Pheochromocytoma/metabolism,physiopathology Rats Rats, Inbred Strains Time Factors Tyrosine 3-Monooxygenase/analysis
Chemicals
Catecholamines Tyrosine 3-Monooxygenase Acetylcholinesterase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jaeger C B
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Article Info
Journal
Experimental brain research
Abbr.
Exp Brain Res
ISSN
0014-4819
DOI
10.1007/BF00261353
Published
1985-00-00
Pages
615-24
Language
English
Region
Germany
NLM ID
0043312
Subset
IM
Grants
NINDS NIH HHS · NS-19699 · United States
External Links
PubMed source DOI: 10.1007/BF00261353 View journal details
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