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PMID: 2859852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Accumulation of inositol phosphates in sympathetic ganglia. Effects of depolarization and of amine and peptide neurotransmitters.

The Biochemical journal ·Vol. 227 ·No. 1 ·1985-04-01 ·Pages 263-9

Bone EA, Michell RH

Abstract

Depolarization of isolated [3H]inositol-labelled rat superior cervical sympathetic ganglia in a high-K+ medium stimulates an accumulation of labelled inositol phosphates. This accumulation occurs only when ganglia are incubated in a Ca2+-containing medium, suggesting that it represents a receptor-stimulated hydrolysis of inositol lipid(s) activated by an endogenously released neurotransmitter. A minor fraction of this accumulation appears to be activated by intraganglionically released acetylcholine, since it is slightly reduced by atropine. The accumulation of inositol phosphates is unaffected by blockade of appropriate catecholamine, histamine and 5-hydroxytryptamine receptors and also by aspirin and indomethacin. This response to depolarization is potentiated by incubation with proteinase inhibitors, suggesting that it might be caused by an endogenously released peptide neutrotransmitter. However, it is not prevented by a V1-vasopressin receptor antagonist, and none of the peptides tested so far fully reproduces the response: these include a stable substance P analogue, physalaemin, neurokinin alpha, bradykinin, angiotensin, pancreozymin, bombesin and luteinizing-hormone-releasing hormone. Stimulated inositol lipid breakdown in depolarized sympathetic ganglia seems likely to be activated by an as-yet-unidentified peptide neurotransmitter: this might serve as an intraganglionic mediator of postsynaptic excitation by employing the same signalling mechanism as muscarinic cholinergic and V1-vasopressin receptors.

MeSH Terms
Animals Atropine/pharmacology Bethanechol Bethanechol Compounds/pharmacology Calcium/pharmacology Ganglia, Sympathetic/drug effects,metabolism In Vitro Techniques Inositol Phosphates/metabolism Muscarine/pharmacology Nerve Tissue Proteins/pharmacology Neurotransmitter Agents/pharmacology Potassium/metabolism Protease Inhibitors/pharmacology Rats Rats, Inbred Strains Sugar Phosphates/metabolism
Chemicals
Bethanechol Compounds Inositol Phosphates Nerve Tissue Proteins Neurotransmitter Agents Protease Inhibitors Sugar Phosphates Bethanechol Atropine Muscarine Potassium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bone E A
Michell R H
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40 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2270263
Published
1985-04-01
Pages
263-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144835
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2270263 PMC Full Text View journal details
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