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PMID: 28528698 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer.

Stem cell reports ·Vol. 8 ·No. 6 ·2017-00-06 ·Pages 1497-1505

Godlewski J, Ferrer-Luna R, Rooj AK, Mineo M, Ricklefs F, Takeda YS, Nowicki MO, Salińska E, Nakano I, Lee H, Weissleder R, Beroukhim R, Chiocca EA, Bronisz A

Abstract

Despite the importance of molecular subtype classification of glioblastoma (GBM), the extent of extracellular vesicle (EV)-driven molecular and phenotypic reprogramming remains poorly understood. To reveal complex subpopulation dynamics within the heterogeneous intratumoral ecosystem, we characterized microRNA expression and secretion in phenotypically diverse subpopulations of patient-derived GBM stem-like cells (GSCs). As EVs and microRNAs convey information that rearranges the molecular landscape in a cell type-specific manner, we argue that intratumoral exchange of microRNA augments the heterogeneity of GSC that is reflected in highly heterogeneous profile of microRNA expression in GBM subtypes.

Keywords

GBM cancer heterogeneity cancer stem cells exosomes extracellular vesicles glioblastoma microRNA subtypes

MeSH Terms

AC133 Antigen/metabolism Animals Brain Neoplasms/genetics,pathology Exosomes/metabolism Extracellular Vesicles/metabolism Female Glioblastoma/genetics,pathology Humans Mice Mice, Nude MicroRNAs/genetics,metabolism Neoplasm Invasiveness Neoplastic Stem Cells/cytology,metabolism Phenotype Tetraspanin 30/metabolism Transcriptome Transplantation, Heterologous Tumor Cells, Cultured

Chemicals

AC133 Antigen MicroRNAs PROM1 protein, human Tetraspanin 30

Authors & Affiliations

14 authors, click to expand affiliations / ORCID

Godlewski Jakub

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: jgodlewski@bwh.harvard.edu.

Ferrer-Luna Ruben

Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Cancer Program, BROAD Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Rooj Arun K

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Mineo Marco

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Ricklefs Franz

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Neurosurgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

Takeda Yuji S

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Nowicki M Oskar

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Salińska Elżbieta

Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences 02-106 Warsaw, Poland.

Nakano Ichiro

Department of Neurosurgery and Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35243, USA.

Lee Hakho

Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Weissleder Ralph

Department of Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Beroukhim Rameen

Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Cancer Program, BROAD Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Chiocca E Antonio

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Bronisz Agnieszka

Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: abronisz@bwh.harvard.edu.

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Article Info

Journal

Stem cell reports

Abbr.

Stem Cell Reports

ISSN

2213-6711

DOI

10.1016/j.stemcr.2017.04.024

jgodlewski@bwh.harvard.edu
abronisz@bwh.harvard.edu

Published

2017-00-06

Epub

2017-00-18

Pages

1497-1505

Language

English

Region

United States

NLM ID

101611300

PMCID

PMC5470095

Subset

Grants

NCI NIH HHS · P01 CA069246 · United States

NCI NIH HHS · R01 CA176203 · United States

NCI NIH HHS · R01 CA183991 · United States

External Links

PubMed source DOI: 10.1016/j.stemcr.2017.04.024 PMC Full Text View journal details

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MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer.

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