Nerve growth factor-induced fiber outgrowth from isolated rat adrenal chromaffin cells: impairment by glucocorticoids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 7 ·1978-07-00 ·Pages 3498-502

Unsicker K, Krisch B, Otten U, Thoenen H

Abstract

Addition of nerve growth factor to cultures of dissociated rat adrenal medullary cells caused fiber outgrowth from chromaffin cells. These fibers exhibited all the characteristics of neurites, particularly the formation of typical growth cones exhibiting intense catecholamine-specific fluorescence. Because this nerve growth factor-mediated neurite outgrowth could be abolished by physiological concentrations of glucocorticoids, it is concluded that the high glucocorticoid concentrations normally present in the adrenal medulla prevent the fiber outgrowth from medullary chromaffin cells in vivo. In dissociated sympathetic neurons the same concentrations of glucocorticoids markedly reduce but do not completely abolish neuronal fiber outgrowth.

MeSH Terms

Adrenal Medulla/drug effects,enzymology,growth & development Animals Cells, Cultured Chromaffin System/drug effects,growth & development Dexamethasone/pharmacology Female Ganglia, Autonomic/drug effects,growth & development Male Nerve Growth Factors/antagonists & inhibitors,pharmacology Rats Tyrosine 3-Monooxygenase/metabolism

Chemicals

Nerve Growth Factors Dexamethasone Tyrosine 3-Monooxygenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Unsicker K

Krisch B

Otten U

Thoenen H

References (19)

19 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.7.3498

Published

1978-07-00

Pages

3498-502

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392805

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.7.3498 PMC Full Text View journal details

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Nerve growth factor-induced fiber outgrowth from isolated rat adrenal chromaffin cells: impairment by glucocorticoids.

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