Home LiteratureArticle Details

PMID: 2851984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Energetics of renal Na+ and H+/L-alanine co-transport systems.

The Biochemical journal ·Vol. 256 ·No. 1 ·1988-11-15 ·Pages 299-302

Jessen H, Vorum H, Jørgensen KE, Sheikh MI

Abstract

The stoichiometric properties of Na+- and H+-dependent L-alanine transporters recently identified in luminal-membrane vesicles prepared from proximal convoluted tubules (pars convoluta) and proximal straight tubules (pars recta) of rabbit kidney were studied. We provide indirect evidence suggesting that one Na+ and one H+ ion are co-transported with the L-alanine molecule via Na+-dependent and H+-dependent transport systems located in vesicles from pars convoluta. Furthermore, our experimental data suggest that both the high-affinity and the low-affinity Na+-dependent L-alanine transport systems of pars recta vesicles operate with a 1:1 stoichiometry.

MeSH Terms

Alanine/pharmacokinetics Animals Biological Transport/drug effects Cell Membrane/drug effects,metabolism Energy Metabolism/drug effects Glucose/metabolism Kidney/drug effects,metabolism Kidney Cortex/metabolism Kidney Medulla/metabolism Protons Rabbits Sodium/pharmacology

Chemicals

Protons Sodium Glucose Alanine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jessen H

Institute of Medical Biochemistry, University of Aarhus, Denmark.

Vorum H

Jørgensen K E

Sheikh M I

References (13)

13 references, click to expand

Glucose transport in isolated brush border membrane from rat small intestine.
J Biol Chem. 1973 Jan 10;248(1):25-32 PMID: 4692832
A simplification of the protein assay method of Lowry et al. which is more generally applicable.
Anal Biochem. 1977 Dec;83(2):346-56 PMID: 603028
Heterogeneity of sodium-dependent D-glucose transport sites along the proximal tubule: evidence from vesicle studies.
Am J Physiol. 1982 Apr;242(4):F406-14 PMID: 6278960
Stoichiometric studies of the renal outer cortical brush border membrane D-glucose transporter.
J Membr Biol. 1982;67(1):73-80 PMID: 7201526
An efficient method for the isolation and separation of basolateral-membrane and luminal-membrane vesicles from rabbit kidney cortex.
Biochem J. 1982 Nov 15;208(2):377-82 PMID: 7159406
Further studies of proximal tubular brush border membrane D-glucose transport heterogeneity.
J Membr Biol. 1982;70(1):37-45 PMID: 7186937
Renal transport of neutral amino acids. Cation-dependent uptake of L-alanine by luminal-membrane vesicles.
Biochem J. 1987 Dec 1;248(2):533-8 PMID: 3435463
Segmental localization of the rabbit renal proximal tubular Na+-H+ exchange system.
Am J Physiol. 1985 Nov;249(5 Pt 2):F704-12 PMID: 4061657
Transport studies in plasma membrane vesicles isolated from renal cortex.
Kidney Int. 1986 Aug;30(2):171-86 PMID: 3531673
Characteristics of D-alanine transport by luminal membrane vesicles from pars convoluta and pars recta of rabbit proximal tubule.
Biochim Biophys Acta. 1988 Jul 21;942(2):262-70 PMID: 3395613
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Mechanism of transport of L-alanine by luminal-membrane vesicles from pars recta of rabbit proximal tubule.
FEBS Lett. 1988 Jan 18;227(1):35-8 PMID: 2828110
Renal transport of neutral amino acids. Tubular localization of Na+-dependent phenylalanine- and glucose-transport systems.
Biochem J. 1984 May 15;220(1):15-24 PMID: 6743259

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2560299

Published

1988-11-15

Pages

299-302

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1135403

Subset

External Links

PubMed source DOI: 10.1042/bj2560299 PMC Full Text View journal details

Analysis Services

Energetics of renal Na+ and H+/L-alanine co-transport systems.

Contact

WeChat Official Account