Inhibition of the proteolysis of rat erythrocyte membrane proteins by a synthetic inhibitor of calpain.

Biochemical and biophysical research communications ·Vol. 157 ·No. 3 ·1988-12-30 ·Pages 1117-23

Mehdi S, Angelastro MR, Wiseman JS, Bey P

Abstract

A synthetic inhibitor of calpain protects rat erythrocyte membrane-associated cytoskeletal proteins from proteolytic degradation (IC50 = 1 microM) which occurs when the cells are rendered permeable to Ca++. Leupeptin, a naturally occurring inhibitor of the enzyme, does not afford any protection at concentrations up to 100 microM.

MeSH Terms

Animals Blotting, Western Calcimycin/pharmacology Calcium/blood Calpain/antagonists & inhibitors Cell Membrane Permeability Cytoskeletal Proteins/blood Dipeptides/pharmacology Erythrocyte Membrane/analysis,drug effects Hydrolysis Leupeptins/pharmacology Membrane Proteins/blood Molecular Weight Peptide Hydrolases/blood Protease Inhibitors Rats Spectrin/metabolism

Chemicals

Cytoskeletal Proteins Dipeptides Leupeptins Membrane Proteins Protease Inhibitors Spectrin Calcimycin Peptide Hydrolases Calpain leupeptin Calcium calpain inhibitor III

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mehdi S

Merrell Dow Research Institute, Cincinnati, OH 45215.

Angelastro M R

Wiseman J S

Bey P

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/s0006-291x(88)80989-6

Published

1988-12-30

Pages

1117-23

Language

English

Region

United States

NLM ID

0372516

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1016/s0006-291x(88)80989-6 View journal details

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Inhibition of the proteolysis of rat erythrocyte membrane proteins by a synthetic inhibitor of calpain.

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