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PMID: 2847252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Leukotriene A4, conversion to leukotriene B4 in human T-cell lines.

Prostaglandins ·Vol. 36 ·No. 2 ·1988-08-00 ·Pages 241-8

Fu JY, Medina JF, Funk CD, Wetterholm A, Rådmark O

Abstract

Human T-cell lines (HSB, MOLT-4 and CCRF-CEM) produced leukotriene B4 when incubated with leukotriene A4. The product was characterized by chromatographic properties, UV-spectroscopy and gas chromatography mass spectrometry. About 10 pmol of leukotriene B4 was obtained per 10(6) cells. When incubated with arachidonic acid plus the calcium ionophore A23187 however, no leukotriene B4 was found, indicating that the T-cell lines lack 5-lipoxygenase yet contain LTA4 hydrolase.

MeSH Terms

Calcimycin/pharmacology Cell Line Gas Chromatography-Mass Spectrometry Humans Leukotriene A4 Leukotriene B4/metabolism Leukotrienes/metabolism T-Lymphocytes/drug effects,metabolism

Chemicals

Leukotriene A4 Leukotrienes Leukotriene B4 Calcimycin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Fu J Y

Dept. of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.

Medina J F

Funk C D

Wetterholm A

Rådmark O

Article Info

Journal

Prostaglandins

Abbr.

Prostaglandins

ISSN

0090-6980

DOI

10.1016/0090-6980(88)90310-3

Published

1988-08-00

Pages

241-8

Language

English

Region

United States

NLM ID

0320271

Subset

External Links

PubMed source DOI: 10.1016/0090-6980(88)90310-3

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Leukotriene A4, conversion to leukotriene B4 in human T-cell lines.

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