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PMID: 2846896 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction and application of plasmids containing bidirectional promoters of vaccinia virus.

Journal of virology ·Vol. 62 ·No. 12 ·1988-12-00 ·Pages 4832-4

Tsao H, Liu GQ, Ruan L, Chu CM

Abstract

A bidirectional expression vector containing both the 11KD late promoter (p11) and the presumptive 25KD early promoter (p25) was constructed. These bidirectional vectors have been applied to the expression of hepatitis B surface antigen by using one of the promoters for beta-galactosidase as the marker gene and the other one for hepatitis B surface antigen as the target gene.

MeSH Terms

DNA Restriction Enzymes Gene Expression Regulation Genetic Markers Genetic Vectors Hepatitis B Surface Antigens/genetics Plasmids Promoter Regions, Genetic Vaccinia virus/genetics beta-Galactosidase/genetics

Chemicals

Genetic Markers Hepatitis B Surface Antigens DNA Restriction Enzymes beta-Galactosidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tsao H

Laboratory of Viral Genetics, Chinese Academy of Preventive Medicine, Beijing, People's Republic of China.

Liu G Q

Ruan L

Chu C M

References (6)

6 references, click to expand

In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
Recovery of DNA fragments inserted by the "tailing" method: regeneration of PstI restriction sites.
Gene. 1981 May;13(4):339-46 PMID: 6266921
Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment.
J Virol. 1986 Nov;60(2):436-49 PMID: 3021979
Transcriptional and translational analysis of a strongly expressed early region of the vaccinia virus genome.
J Virol. 1984 Feb;49(2):459-70 PMID: 6319745
Gene coding for the late 11,000-dalton polypeptide of the Tian Tan strain of vaccinia virus and its 5'-flanking region: nucleotide sequence.
J Virol. 1986 Feb;57(2):693-6 PMID: 3944848
Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA.
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1210-4 PMID: 6280173

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.62.12.4832-4834.1988

Published

1988-12-00

Pages

4832-4

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253610

Subset

External Links

PubMed source DOI: 10.1128/JVI.62.12.4832-4834.1988 PMC Full Text View journal details

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Construction and application of plasmids containing bidirectional promoters of vaccinia virus.

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