Separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium Paracoccus denitrificans and their occurrence in other denitrificans bacteria.

The Biochemical journal ·Vol. 252 ·No. 3 ·1988-06-15 ·Pages 905-8

Kucera I, Hedbávný R, Dadák V

Abstract

By means of the method of fluorimetric titration it has been shown that mucidin does not affect the attachment of antimycin to membranes from anaerobically grown Paracoccus denitrificans. The fluorimetric titration with antimycin can be used in the determination of the amount of the cytochrome bc1 complex in the membrane. In cells inhibited with antimycin, the oxidation of cytochromes c was accompanied by the reduction of cytochrome b; in the presence of mucidin this effect did not take place. The results, which indicated a difference in binding sites, were interpreted in terms of the Q-cycle [Mitchell (1976) J. Theor. Biol. 62, 327-367; Trumpower (1981) Biochim. Biophys. Acta 639, 129-155]. Comparable sensitivity towards antimycin and mucidin was shown by other typical denitrifying bacteria: Pseudomonas stutzeri and Alcaligenes xylosoidans, subspecies denitrificans.

MeSH Terms

Alcaligenes/metabolism Alkenes/metabolism Antimycin A/analogs & derivatives,metabolism Binding Sites Cell Membrane/metabolism Cytochromes/metabolism Electron Transport Electron Transport Complex III/metabolism Fatty Acids, Unsaturated Methacrylates Nitrite Reductases/antagonists & inhibitors Oxidation-Reduction Oxygen Consumption Paracoccus denitrificans/metabolism Pseudomonas/metabolism Spectrometry, Fluorescence Strobilurins

Chemicals

Alkenes Cytochromes Fatty Acids, Unsaturated Methacrylates Strobilurins antimycin mucidin Antimycin A Nitrite Reductases Electron Transport Complex III

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kucera I

Department of Biochemistry, Faculty of Science, J. E. Purkynĕ University, Brno, Czechoslovakia.

Hedbávný R

Dadák V

References (13)

13 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2520905

Published

1988-06-15

Pages

905-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1149234

Subset

External Links

PubMed source DOI: 10.1042/bj2520905 PMC Full Text View journal details

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Separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium Paracoccus denitrificans and their occurrence in other denitrificans bacteria.

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