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PMID: 2844150 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Plant holo-(acyl carrier protein) synthase.

The Biochemical journal ·Vol. 252 ·No. 1 ·1988-05-15 ·Pages 39-45

Elhussein SA, Miernyk JA, Ohlrogge JB

Abstract

1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The partially purified enzyme had a pH optimum of 8.2 and Km values of 2 microM, 72 microM and 3 mM for apo-(acyl carrier protein), CoA and Mg2+ respectively. Synthase activity was inhibited in vitro by the reaction product 3',5'-ADP. 4. Results from the fractionation of spinach leaf and developing castor-oil-seed (Ricinus communis) endosperm cells were consistent with a cytosolic localization of holo-(acyl carrier protein) synthase activity in plant cells.

MeSH Terms
Acyl Carrier Protein/metabolism Adenosine Diphosphate/pharmacology Chromatography, Gel Chromatography, Ion Exchange Coenzyme A/metabolism Magnesium/metabolism Magnesium Chloride Phosphotransferases/antagonists & inhibitors,isolation & purification,metabolism Plants/enzymology Subcellular Fractions/enzymology Transferases (Other Substituted Phosphate Groups)
Chemicals
Acyl Carrier Protein Magnesium Chloride Adenosine Diphosphate Phosphotransferases Transferases (Other Substituted Phosphate Groups) holo-(acyl-carrier-protein) synthase Magnesium Coenzyme A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elhussein S A
Seed Biosynthesis Research Unit, United States Department of Agriculture, Peoria, IL 61604.
Miernyk J A
Ohlrogge J B
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20 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2520039
Published
1988-05-15
Pages
39-45
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1149104
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2520039 PMC Full Text View journal details
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