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PMID: 284393 Published · ppublish English Journal Article

Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 2 ·1979-02-00 ·Pages 700-4

Belin D, Hedgpeth J, Selzer GB, Epstein RH

Abstract

We have determined the sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo. The rIIB mutant HD263, which is temperature sensitive for translation, has an altered initiation codon (AUA instead of AUG). Because wild-type and HD263 proteins have the same fMet peptide, this AUA is used to initiate translation in the mutant rIIB mRNA. We have also identified a six-base sequence which is complementary to the 3" end of the 16S rRNA.

MeSH Terms
Base Sequence Binding Sites Codon Coliphages/genetics Genes, Viral Mutation Peptide Chain Initiation, Translational RNA, Messenger/genetics Ribosomes/metabolism Temperature
Chemicals
Codon RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Belin D
Hedgpeth J
Selzer G B
Epstein R H
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.2.700
Published
1979-02-00
Pages
700-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383023
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.2.700 PMC Full Text View journal details
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