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PMID: 284347 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Procollagen segment-long-spacing crystallites: their role in collagen fibrillogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 1 ·1979-01-00 ·Pages 313-7

Bruns RR, Hulmes DJ, Therrien SF, Gross J

Abstract

Naturally occurring segment-long-spacing crystallites of procollagen collagen have been found in the culture medium of fibroblasts from chick embryo tendon, human skin, and dermatosparaxic calf skin; in whole-mount preparations of cultured human skin fibroblasts; in homogenates of lathyritic chick embryo tendon, cartilage, and cornea; and in a partially purified preparation of procollagen. Bundles of similar aggregates occurred within secretory vacuoles of collagen-synthesizing fibroblasts and chondrocytes. These observations suggest that fibroblasts and chondrocytes secrete procollagen assemblies that are stable in the extracellular environment. We propose that subsequent enzymatic processing is accompanied by direct incorporation of such structures into the assembling fibril, which then may be considered as an n X 67 nm staggered array of segment-long-spacing crystallites.

MeSH Terms
Animals Cattle Chick Embryo Collagen/metabolism Cornea/ultrastructure Crystallization Cytoplasmic Granules/ultrastructure Fibroblasts/ultrastructure Humans Lathyrism/pathology Procollagen/metabolism Protein Binding Skin/ultrastructure Tendons/ultrastructure
Chemicals
Procollagen Collagen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bruns R R
Hulmes D J
Therrien S F
Gross J
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.1.313
Published
1979-01-00
Pages
313-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC382929
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.1.313 PMC Full Text View journal details
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