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PMID: 2843467 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human bactericidal antibody response to outer membrane protein P2 of nontypeable Haemophilus influenzae.

Infection and immunity ·Vol. 56 ·No. 10 ·1988-10-00 ·Pages 2673-9

Murphy TF, Bartos LC

Abstract

The human bactericidal antibody response to the major outer membrane protein, P2, of nontypeable Haemophilus influenzae was studied. P2 was isolated from two strains of nontypeable H. influenzae and coupled to affinity columns. Pooled normal human serum was subjected to affinity chromatography with the P2 columns and a control column. Reducing the titer of antibody to P2 resulted in reduced bactericidal activity of that serum for the organism. Immunopurified antibody to P2 from human serum was bactericidal for the homologous strain. The extent to which these bactericidal determinants on P2 are conserved among strains was investigated. Immunopurified antibodies to P2 of two epidemiologically unrelated isolates were bactericidal for four of six strains tested. We conclude that P2 is a target for human bactericidal antibody and that some of these determinants that are recognized by human bactericidal antibody are conserved among strains of nontypeable H. influenzae.

MeSH Terms
Antibodies, Bacterial/immunology Antigens, Bacterial/immunology Bacterial Outer Membrane Proteins/immunology Blood Bactericidal Activity Chromatography, Affinity Enzyme-Linked Immunosorbent Assay Haemophilus influenzae/immunology Humans Lipopolysaccharides/immunology Molecular Weight Porins
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Outer Membrane Proteins Lipopolysaccharides Porins lipid-linked oligosaccharides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murphy T F
Department of Medicine, State University of New York, Buffalo 14215.
Bartos L C
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.10.2673-2679.1988
Published
1988-10-00
Pages
2673-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259628
Subset
IM
Grants
NIAID NIH HHS · AI 19641 · United States
External Links
PubMed source DOI: 10.1128/iai.56.10.2673-2679.1988 PMC Full Text View journal details
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