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PMID: 2843166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effect of the NADPH oxidase inhibitor diphenyleneiodonium on aerobic and anaerobic microbicidal activities of human neutrophils.

The Biochemical journal ·Vol. 251 ·No. 3 ·1988-05-01 ·Pages 887-91

Ellis JA, Mayer SJ, Jones OT

Abstract

NADPH-dependent superoxide production by intact human neutrophils is inhibited by DPI (diphenyleneiodonium), when stimulated by either FMLP (N-formylmethionyl-leucyl-phenylalanine) or PMA (phorbol 12-myristate 13-acetate). Addition of 10 microM-DPI abolished the reduction of both the FAD and the cytochrome b components of the NADPH oxidase. DPI inhibition of the oxidase was associated with defective aerobic killing of staphylococci by human neutrophils. Anaerobic killing, phagocytosis, chemotaxis and motility were relatively unaffected by 10 microM-DPI. Degranulation of the azurophil and specific granules, induced by the soluble stimuli FMLP or PMA, and by particulate stimuli was decreased by the presence of DPI. The above effects of DPI on human neutrophils are similar to those found in chronic granulomatous disease.

MeSH Terms
Aerobiosis Anaerobiosis Blood Bactericidal Activity/drug effects Cell Movement/drug effects Cell Survival/drug effects Humans In Vitro Techniques N-Formylmethionine Leucyl-Phenylalanine/pharmacology NADH, NADPH Oxidoreductases/antagonists & inhibitors NADPH Oxidases Neutrophils/drug effects,enzymology,physiology Onium Compounds/pharmacology Spectrophotometry Superoxides/blood Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Onium Compounds Superoxides N-Formylmethionine Leucyl-Phenylalanine diphenyleneiodonium NADH, NADPH Oxidoreductases NADPH Oxidases Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ellis J A
Department of Biochemistry, Medical School, University of Bristol, U.K.
Mayer S J
Jones O T
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2510887
Published
1988-05-01
Pages
887-91
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1149085
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2510887 PMC Full Text View journal details
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